https://revistas.censa.edu.cu/index.php/RPV/issue/feed Revista de Protección Vegetal 2025-07-09T22:54:08+00:00 Dra. C. Mayra G. Rodríguez Hernández revistadeproteccionvegetal@gmail.com Open Journal Systems <p align="justify"><span style="font-family: verdana; font-size: small;">Revista de Protección Vegetal, an organ of scientific diffusion of the National Center for Animal and Plant Health (CENSA), publishes research results in Plant Protection of this and other national and foreign institutions under <a href="https://creativecommons.org/licenses/by-nc/4.0/deed.es_ES" target="_blank" rel="noopener">License Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)</a>. It is addressed to professionals and technicians related to this activity and related sciences. It has been published uninterruptedly since 1986 and currently has a continuous publication system consisting of one volume per year. Diffuse researches on Plant Health. The main topics are: Diagnostic and identification of harmful organisms in plants; Biology and populational studies; Pest and disease management; Development of biological control agents; as well as works related to the Characterization of pathogens and Resistance to pests and diseases. Topics related to Quality management are also included. The Editorial Board will welcome contributions related to Plant Protection, referring to: diagnosis, identification and characterization of organisms harmful to plants, bioinformatics applied to Plant Protection, biology and population studies of pests, integrated and agroecological management of pests, chemical ecology, development and use of biological control agents, multitrophic interactions and pest resistance, studies of damages caused by pests to crops and stored agricultural products, socio-economic studies related to pest management, quality management in the manufacture of microbial pesticides and in the mass production of beneficial arthropods, mathematics and informatics applied to Plant Health, methods of pesticide application, environmental impact of pesticide use and benefits of their rational use, field trials of genetically modified crops, invasive pests, climate change and its relationships with pests and biorregulators, agricultural extension, studies of hazards, and vulnerabilities and risks of phytosanitary disasters. The manuscripts, technically valid, will be published in Spanish and English, with a summary in both languages. Authors whose mother tongue is Spanish must submit the manuscript in that language; the journal will be responsible for its translation into the English language. The contributions are anonymously peer reviewed by specialists from various national and international institutions that guarantee the technical quality and content of the journal. The results are published in different formats: Original Article, Short Communication, Letter to the Editor, Review Article, and Technical Note.</span></p> https://revistas.censa.edu.cu/index.php/RPV/article/view/1377 Genesis and evolution of Plant Health at the Indio Hatuey Pasture and Forage Experimental Station, Matanzas, Cuba 2025-06-04T02:39:13+00:00 Osmel Alonso Amaro osmel@ihatuey.cu Juan Carlos Lezcano Fleires osmel@ihatuey.cu Roberto Miret Alfonso osmel@ihatuey.cu Antonio Delgado Perdomo osmel@ihatuey.cu <p>Plant protection, together with plant nutrition, are two of the most important cultural practices in productive systems, even more if they are destined to animal production, when they are long term systems. Thus, the “phytosanitary” topic has been present in the Experimental Station of Pastures and Forages Indio Hatuey (EEPFIH) since its foundation in 1962. In this regard, the objective of this paper was to review the scientific work and the contribution of this topic to the country by researchers of the station. The insertion of the topic into the science and technology plan started when the pest indicator was taking into account in the evaluation of the grassland and forages species to be approved as commercial varieties until arriving, at present, at the determination of the evolution of agroecosystems in the forest context, with a marked diversity of plant and animal species and the use of horizontal and vertical space, to secure the existence of microenvironments able to contribute to natural enemy conservation and creation of seminatural forests where no massive pest outbreaks occur and there is a stable biological balance. Hence, it is concluded that the topic of plant protection in the EEPFIH is on the right path in function of the crop and the productive system, on agroecological basis; similar to what happens in the country in the transformation process from conventional agriculture to agriculture in agroecological transition, which is seen as the challenge to maintain in the immediate future.</p> 2025-06-04T00:00:00+00:00 Copyright (c) 2025 https://revistas.censa.edu.cu/index.php/RPV/article/view/1405 Contributions to the topic of entomopathogenic nematodes by institutions of higher education in Cuba 2025-07-07T20:13:12+00:00 Mayra G. Rodríguez Hernández mayrag2531961@gmail.com <p>The objective of this work was to summarize the contributions to the entomopathogenic nematode (EPN) development and use as biological control agents in the social practice in Cuba by researchers and professors from the Ministry of Higher Education (MES). More than 80 articles and book chapters published in the country and abroad were reviewed. The National Center for Plant and Animal Health (CENSA) and the Central University "Marta Abreu" of Las Villas (UCLV) have been the greatest contributors since the mid-90s. Numerous pest insects of the orders Lepidoptera, Coleoptera, and Homoptera were studied under semi-controlled conditions in the laboratory and, to a lesser extent, in the field to determine their susceptibility to EPN, especially to various species and strains of the genus <em>Heterorhabditis</em>. Effective results were obtained when important pests of food-security crops, such as corn (<em>Zea mays </em>L.) and sweet potato (<em>Ipomoea batantas </em>(L.) Lam), and others with relevance as exportable items, such as tobacco (<em>Nicotiana tabacum </em>L.) and coffee (<em>Coffea </em>spp.), were treated with EPN in the field, and the studied EPN species and strain were incorporated as biological control agents in management programs. The potential to establish working alliances on various issues related to the development and efficient use of EPN in Cuba, to which MES specialists can contribute, was made evident, and so were the challenges of the subject, which could be research objectives in university-business alliances.</p> 2025-06-30T00:00:00+00:00 Copyright (c) 2025 Este artículo se encuentra bajo licencia Creative Commons Reconocimiento-NoComercial 4.0 Internacional (CC BY-NC 4.0) https://revistas.censa.edu.cu/index.php/RPV/article/view/1391 Studies at the University of Cienfuegos "Carlos R. Rodriguez", Cuba, for the management of mollusc pests in agroecosystems 2025-06-26T17:14:11+00:00 Maité Nodarse Castillo mnodarse@ucf.edu.cu Wendy Gómez Menéndez mnodarse@ucf.edu.cu <p>The production of vegetables in organoponics is a priority in the development of urban agriculture in Cuba. One of the main problems associated with this is the damage caused by pests, among which the phytophagous mollusks stand out. This cropping system does not allow using synthetic pesticides for pest control. Therefore, it has been a priority task for the country to seek alternatives such as the application of biopesticides for an agroecological management of mollusks. This work shows the results achieved in this field of research at the Universidad de Cienfuegos. It was developed and made available to the academic and productive community a guide for the identification of mollusk species of agricultural interest present in the organoponics of the province, such as <em>Subulina octona </em>(Bruguiére), <em>Praticolella griseola </em>(Pfeiffer), <em>Bradybaena similares </em>(Ferrusac), <em>Zachrysia auricoma </em>(Ferrusac), <em>Leidyula floridana </em>(Leidy &amp; Binney in Binney) and <em>Oleacina estraminea </em>(Deshaye), and <em>Huttonella bicolor </em>(Hutton). In addition, it was proposed to use plant genetic resources with wide distribution in the national territory, among which are different species of the Agavaceae family (<em>Agave americana </em>L., <em>Agave legrelliana </em>Jacobi and <em>Furcraea hexapetala </em>Urban), for management.</p> 2025-03-18T00:00:00+00:00 Copyright (c) 2025 Este artículo se encuentra bajo licencia Creative Commons Reconocimiento-NoComercial 4.0 Internacional (CC BY-NC 4.0) https://revistas.censa.edu.cu/index.php/RPV/article/view/1392 Frequently occurring errors in agricultural research due to the incorrect use of statistical methods 2025-06-26T17:26:46+00:00 Mario Varela Nualles varela@inca.edu.cu Lucía Fernández Chuairey varela@inca.edu.cu José Antonio Pino Roque varela@inca.edu.cu Ileana Miranda Cabrera varela@inca.edu.cu <p>The objectives of this article were I) to warn about recurrent statistical errors that are frequently found in different scientific papers in agricultural research and associated with conceptual aspects and the misuse of computational tools, specifically, in the context of the analysis of variance (ANOVA) and II) provide work methodologies that allow the correct use of these statistical procedures. Four types of errors are approached: an error associated with the assumption of normality; a second one linked to non-parametric multiple comparison tests; another error consists of not considering a single standard error when reporting confidence intervals in an ANOVA context; and the fourth error is related to the interpretation of the p-value when it is very close to the level of significance. An example is included and shows how to proceed by using statistical software. In general, criteria and work methodologies are established that favor the correct use of these procedures. Taking into consideration the aspects approached in this work will make it possible to avoid issuing biased conclusions, which will contribute to obtaining significant and valuable information from the results in research processes and scientific papers.</p> 2025-04-09T00:00:00+00:00 Copyright (c) 2025 Este artículo se encuentra bajo licencia Creative Commons Reconocimiento-NoComercial 4.0 Internacional (CC BY-NC 4.0) https://revistas.censa.edu.cu/index.php/RPV/article/view/1394 Doctoral training in Plant Health at the National Center for Animal and Plant Health: a contribution to postgraduate capacity building in the present context 2025-06-26T18:23:05+00:00 Moraima Suris Campos bpeteira@censa.edu.cu Sandra Cuello Portal bpeteira@censa.edu.cu Belkis Peteira Delgado-Oramas bpeteira@censa.edu.cu <p>The objective of this work was to present the experiences of a Science, Technology and Innovation Entity (ECTI) of the Ministry of Higher Education (MES) in the training of highly qualified specialists in Plant Health. Today's agriculture requires a sustainable increase in yields to be guaranteed at a lower economic, environmental and social cost. For this, it is essential to master the biological complexities of the agents that affect the different agroecosystems, a basis on which pest management tactics must be based so that the most appropriate ones are applied taking into account the ability to manipulate their intrinsic characteristics, as well as the environment in which they develop. Training of highly qualified specialists is needed, which is a priority objective of the National System of Scientific Degrees (SNGC). Training of Dr. C. is developed within the framework of the Doctoral Program in Plant Health, which began in January 2019 with the purpose of training Doctors in Agricultural Sciences with an updated vision of the national and international challenges, conditioned by climate change, the protection of the human health, the environment, and food safety, with the novel “One Health” approach. This program provides for the strengthening of the theoretical-practical relationship so that the profile of the graduate is shaped for the development of skills that link the theoretical knowledge acquired and its application in productive practice. Details of the operation of the current Program in Plant Health are provided.</p> 2025-02-04T00:00:00+00:00 Copyright (c) 2025 Este artículo se encuentra bajo licencia Creative Commons Reconocimiento-NoComercial 4.0 Internacional (CC BY-NC 4.0) https://revistas.censa.edu.cu/index.php/RPV/article/view/1403 Taxonomy, biology, and ecology of phytophagous mites (Acari: Trombidiformes) in Cuba 2025-07-01T14:37:47+00:00 Mayra Ramos Lima ramosmayra@itmexicali.edu.mx Héctor Rodríguez Morell ramosmayra@itmexicali.edu.mx Leandra Amabela Ramírez Quintero ramosmayra@itmexicali.edu.mx Geyser Flores Galano ramosmayra@itmexicali.edu.mx Adrián Montoya Ramos ramosmayra@itmexicali.edu.mx Delvy Alonso Rodríguez ramosmayra@itmexicali.edu.mx Marbely del Toro Benítez ramosmayra@itmexicali.edu.mx Adayakni Sánchez Castro ramosmayra@itmexicali.edu.mx Reynaldo Chico Morejón ramosmayra@itmexicali.edu.mx <p>Several species of Trombidiform phytophagous mites are a serious threat for agricultural production because of the deleterious effects on crop yields by their feeding on plants. They have been studied on crops of interest such as citrus (<em>Citrus</em>&nbsp;spp.), plantain (<em>Musa</em>&nbsp;spp.), potato (<em>Solanum tuberosum</em>&nbsp;L.), and rice (<em>Oryza sativa</em>&nbsp;L.), on which their negative impact on yield is evident. The combination of taxonomic, biological, ecological and population studies of the endemic mite species&nbsp;<em>Tetranychus urticae</em>&nbsp;(Koch),&nbsp;<em>Panonychus citri</em>&nbsp;(Mc Gregor),&nbsp;<em>Tetranychus tumidus</em>&nbsp;Banks<em>, Polyphagotarsonemus latus</em>&nbsp;(Banks), as well as of the exotic species&nbsp;<em>Steneotarsonemus spinki</em>&nbsp;Smiley and&nbsp;<em>Raoiella indica</em>&nbsp;Hirst, offered the scientific bases to support the management proposals adopted by the country. The present review is aimed to summarize the main results obtained in Cuba by science entities and universities from Higher Education institutions.</p> 2025-06-17T00:00:00+00:00 Copyright (c) 2025 Este artículo se encuentra bajo licencia Creative Commons Reconocimiento-NoComercial 4.0 Internacional (CC BY-NC 4.0) https://revistas.censa.edu.cu/index.php/RPV/article/view/1374 In vitro effect of Cuban strains of Trichoderma spp. on the growth of the pathogen Phyllosticta citricarpa (McAlpine) Aa 2025-06-03T23:14:37+00:00 Ana M. Manzano León anamargaritamanzanoleon@gmail.com Wendy Serra anamargaritamanzanoleon@gmail.com Amalia Sanz Llorente anamargaritamanzanoleon@gmail.com Amaia Ponce de la Cal anamargaritamanzanoleon@gmail.com Yamilé Baró Robaina anamargaritamanzanoleon@gmail.com Frank A. Díaz Leyva anamargaritamanzanoleon@gmail.com <p>The objective of this research was to evaluate the <em>in vitro </em>effect of <em>Trichoderma </em>spp. on <em>Phyllosticta citricarpa </em>(McAlpine) Aa growth. A total of twenty <em>P. citricarpa </em>isolates from major citrus-producing regions in Cuba were included in the analysis. <em>Trichoderma harzianum </em>Rifai (strains LBAT-34 and LBAT-53) and <em>Trichoderma viride </em>Pers. (strain LBAT-TS3) were tested for their ability to inhibit mycelial growth of <em>P. citricarpa. </em>Inhibition was evaluated using the <em>in vitro </em>dual culture assay. Percentages of inhibition of radial growth (PIRG) were determined after 3, 5, and 7 days, and type and intensity of the antagonism of <em>Trichoderma </em>were also classified. Kruskal-Wallis and non-parametric multiple comparison tests were implemented for the statistical analysis. Results showed that the percentages of inhibition of radial growth ranged from 38.7% to 29.2% after 3 days of co-culture. After the fifth day, the antagonist strains overran the <em>P. citricarpa </em>colonies and inhibited their growth completely. Furthermore, it was found that the mycelial growth inhibition of <em>P. citricarpa </em>isolates by <em>Trichoderma </em>spp. was through mechanisms of antibiosis and hyperparasitism. This study is the first evaluation of the susceptibility of <em>P. citricarpa </em>to biological control agents in Cuba.</p> 2025-06-03T00:00:00+00:00 Copyright (c) 2025 This is an open access article under terms of the license Creative Commons Attribution-NonCommercial (BY-NC 4.0) https://revistas.censa.edu.cu/index.php/RPV/article/view/1400 Phytopathogenic agents associated with Plukenetia volubilis L. in Cuba: symptomatology and observations about their severity and degree of infestation 2025-07-01T13:57:22+00:00 Mabel Villanueva-Domínguez mvillanueva@bionaturasm.cu Emilio Fernández-Gonzálvez mvillanueva@bionaturasm.cu Reinaldo Israel Cabrera-Cabrera mvillanueva@bionaturasm.cu Keily Montero-Alvarez mvillanueva@bionaturasm.cu Ernesto Cardoso-Hernández mvillanueva@bionaturasm.cu María del Carmen Pérez-Hernández mvillanueva@bionaturasm.cu Miguel Ramos-Leal mvillanueva@bionaturasm.cu <p>The objectives of this work were to determine the presence of phytopathogens in important producing areas of Sacha Inchi (<em>Plukenetia volubilis</em>&nbsp;L.) in Cuba and provide some observations of their severity and degree of the crop infestation. The soil and root samples analyzed were from plantations in three different areas of the western region, two from the province of Mayabeque and one from Pinar del Rio. In the field, areas of poorly developed and dead plants were found. The roots of these plants showed several damages, such as corky areas even up to the level of the plant neck, scarce secondary system, galls of variable size, and, in occasions, tumors with or without necrosis. The phytosanitary diagnosis allowed the detection of the nematode species&nbsp;<em>Meloidogyne incognita</em>&nbsp;(Kofoid &amp; White) Chitwood,&nbsp;<em>Meloidogyne arenaria</em>&nbsp;(Neal) Chitwood,&nbsp;<em>Trichodorus</em>&nbsp;spp.,&nbsp;<em>Helicotylenchus dihystera</em>&nbsp;(Cobb) Sher,&nbsp;<em>Aphelenchoides bicaudatus</em>&nbsp;(Inamura),&nbsp;<em>Tylenchorhynchus</em>&nbsp;spp., and&nbsp;<em>Xiphinema</em>&nbsp;spp., predominating&nbsp;<em>M. incognita</em>. The fungi&nbsp;<em>Fusarium</em>&nbsp;spp. and&nbsp;<em>Lasiodiplodia</em>&nbsp;spp. was also observed. The combined effect of the&nbsp;<em>Meloidogyne</em>-<em>Fusarium</em>&nbsp;complex damaged Sacha Inchi seriously with important consequences for its production.</p> 2025-05-26T00:00:00+00:00 Copyright (c) 2025 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial (CC BY-NC 4.0). https://revistas.censa.edu.cu/index.php/RPV/article/view/1411 Modeling Spodoptera frugiperda larvae J. E. (Smith) density in corn (Zea mays L.) in Mayabeque Province, Cuba 2025-07-09T22:19:48+00:00 Josefina V. Gómez Piñar ileanam@censa.edu.cu Yaisys Blanco Valdés ileanam@censa.edu.cu Avline Duvelsaint ileanam@censa.edu.cu Neisy Castillo Reyes ileanam@censa.edu.cu Ileana Miranda Cabrera ileanam@censa.edu.cu <p>With the objective of estimating the density of <em>Spodoptera frugiperda</em> J. E. (Smith) larvae in an experimental corn (<em>Zea mays</em> L.) area, a mathematical model that described the population-climate relationship was implemented. To develop the model, the data were taken from samplings carried out from day 28 after the crop emergence to harvest in an experimental area of ​​0.1 ha of the commercial corn cultivar “Francisco” from INIFAT. The interior of the buds on 80 randomly selected plants was examined by traveling across the field in a zigzag pattern.&nbsp;&nbsp; In each sampling, the f plants with <em>S. frugiperda</em> larvae were counted and the density found recorded. The curves of infestation percentage and average density of larvae were described. It was developed a linear regression model that estimated larval density based on days after emergence, average temperature, solar radiation, relative humidity, wind speed, and accumulated precipitation. It was estimated that in the town of Mayabeque, Cuba, in2030, the average temperature will remain between 22 and 25 °C in the months of February, March and April, but rainfall will show a considerable decrease, which will influence the increase in insect populations. A validation of the proposed model in other locations, for this and other cultivars, will provide a tool for predicting the larval density of <em>S. frugiperda</em> in future climate scenarios.</p> 2025-07-09T00:00:00+00:00 Copyright (c) 2025 https://revistas.censa.edu.cu/index.php/RPV/article/view/1412 Control of powdery mildew on pumpkin (Cucurbita pepo L.) under greenhouse conditions 2025-07-09T22:32:49+00:00 Jorge Francisco León de la Rocha jfrleeon@gmail.com Nazario Francisco Francisco jfrleeon@gmail.com Juan Antonio Juárez Cortez jfrleeon@gmail.com Humberto Rafael Bravo Delgado jfrleeon@gmail.com Irma García Cruz jfrleeon@gmail.com Yusimy Reyes Duque jfrleeon@gmail.com <p>The objective of this study was to define the optimal concentrations and timing of applications of oils, mineral salts, and biological control agents for powdery mildew management in squash (<em>Cucurbita pepo</em> L. cv. ‘Grey Zucchini’) under greenhouse conditions. After seven days of seed germination, the true leaves of the seedlings were removed and only the cotyledons remained. They were then inoculated with the pathogenic fungus, and three concentrations of the products were applied at two time: preventively or curatively. At the doses evaluated, the preventive applications of any of the products (oils, mineral salts, or biological control agents) showed some effect on the disease, and the disease severity did not significantly differ from the control. Compared with the control, which showed 80.93%, the curative treatments showed 41.67% and 71.43% disease severity with significant differences at medium concentrations for oils and low concentrations for salts (10 ml.L<sup>-1</sup>) and (2 g.L<sup>-1</sup>), respectively. On this basis, the curative applications for oils and salts were selected, with the intermediate concentration (10 ml.L<sup>-1</sup>) for the former and the minimum concentration for the latter (2 g.L<sup>-1</sup>, 2 ml.L<sup>-1</sup>).</p> 2025-07-09T00:00:00+00:00 Copyright (c) 2025 https://revistas.censa.edu.cu/index.php/RPV/article/view/1413 Nematicidal effect of essential oils from Lamiaceae family against Meloidogyne incognita (Kofoid & White) Chitwood 2025-07-09T22:54:08+00:00 Oriela Pino Pérez oriela@censa.edu.cu Mayra G. Rodríguez Hernández oriela@censa.edu.cu Daine Hernández-Ochandía oriela@censa.edu.cu Roberto Enrique Regalado oriela@censa.edu.cu Beatriz Álvarez Pita oriela@censa.edu.cu Annie Rubio Ortega oriela@censa.edu.cu Yaima Sánchez Pérez oriela@censa.edu.cu María C. Travieso Novelles oriela@censa.edu.cu Cecil González Suárez oriela@censa.edu.cu Susana Gorrita Ramírez oriela@censa.edu.cu Belkis Peteira Delgado-Oramas oriela@censa.edu.cu Teresa M. Correa Vidal oriela@censa.edu.cu Rodny Montes de Oca Porto oriela@censa.edu.cu Rafael Deroncelé Caignet oriela@censa.edu.cu <p>The aim of this study was to determine the effect of eight essential oils from plants belonging to the Lamiaceae family on <em>Meloidogyne incognita</em> (Kofoid &amp; White) Chitwood. The essential oils were obtained from <em>Mentha arvensis</em> L., <em>Mentha piperita</em> L., <em>Mentha spicata</em> L., <em>Ocimum basilicum</em> var <em>genovese</em> L., <em>Ocimum gratisimum</em> L., <em>Rosmarinus officinalis</em> L., and <em>Thymus vulgaris</em> L. by hydrodistillation in a Clevenger equipment. The biological activity in three populations of the nematode was evaluated by a 96-microwell plate immersion bioassay. The chemical composition of the oils was determined by Gas Chromatography/Mass Spectrometry (CG/EM). The yields of the essential oils obtained were between 0.65 and 0.10% (v/m). All oils showed toxicity on <em>M. incognita</em> juveniles although the number of them with nematicidal effect varied for each population. The treated nematodes showed damaged in internal structures: a disorganized digestive system, large vacuole-like spaces along the body, a deformed intestine, and the presence of granules. The oils from <em>O. tenuiflorum</em> and <em>M. arvensis</em> were notable for causing high mortality rates in the three <em>M. incognita</em> populations evaluated. In the population from tomato (<em>Solanum lycopersicum</em> L.), the LC<sub>50</sub> of the <em>M. arvensis</em> oil was 0.141% (0.126-0.163) and it was estimated as 0.118% for <em>O. tenuiflorum</em> after 24 hours. The analysis by GC/MS of the chemical composition of the essential oils studied revealed the presence of monoterpenoids, sesquiterpenoids and phenylpropanoids. The major components in the two oils with the highest activity were linalool (65.59%), α-terpineol (10.42%), eugenol (41.10%), β-caryophyllene (25.04%), and β-elemene (22.42%). The nematotoxic effect shown by these oils, especially those of <em>O. tenuiflorum</em> and <em>M. arvensis</em>, provides the basis for the development of new nematicides and alternative methods for nematode management in different crops of importance in Cuban agriculture.</p> 2025-07-09T00:00:00+00:00 Copyright (c) 2025