Desarrollo embrionario de bovino in vitro cocultivado con células oviductales y del cumulus oophorus

Yury Grisel Soto-Martínez, Eduardo Casas-Hernández, José Miguel Betancourt-Rule, Filiberto Fernández-Reyes

Resumen

La presente investigación tuvo como objetivo evaluar el efecto del cocultivo de los embriones con células oviductales y del cúmulo oóforo (cumulus oophorus) sobre el desarrollo de embriones in vitro en estadio de blastocisto. Los oviductos y ovarios se colectaron de hembras bovinas sacrificadas en un rastro. Las células oviductales se obtuvieron tras una incisión longitudinal del oviducto; las células del cumulo oophorus se colectaron del líquido folicular. Ambos tipos celulares se colocaron para su cultivo en TCM-199 con 20 % de Suero Fetal Bovino (SFB). Los complejos cúmulo-ovocitos (COC’s) fueron colectados por aspiración de folículos de 2 a 7 mm de diámetro y colocados para su maduración en TCM-199 con 10 % de SFB; posteriormente, se fertilizaron utilizando una concentración de 1x106 espermatozoides/mL. Después de la fertilización se dividieron en tres tratamientos: 1) Control, 2) Cocultivo con células de cúmulo, 3) Cocultivo con células del oviducto. El desarrollo se evaluó a 192 horas de cultivo in vitro, determinando la cantidad de embriones divididos, mórulas y blastocistos. Se cultivaron en medio SOF secuencial, por tratamiento: 1) 538 ovocitos, 2) 627 ovocitos y 3) 478 ovocitos. Los resultados fueron los siguientes: 14 % de blastocistos en el cocultivo de células cúmulo, 7 % en el cocultivo de oviducto y 4 % en el control, con una diferencia estadística significativa (p<0,05) entre los tres tratamientos. Se concluye que el uso de medios condicionados con células somáticas puede estimular el desarrollo in vitro hasta estadio de blastocisto de los embriones bovinos.

Palabras clave

desarrollo embrionario; bovino; in vitro; cocultivo

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Referencias

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