Antimicrobial susceptibility and biofilm formation in Escherichia coli isolates from laying hens
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Abstract
Escherichia coli is an important pathogen in poultry associated with enteric and respiratory processes, causing significant losses due to the decrease in the laying and costs in medicines. Plasmids and chemosomes encoding resistance mechanisms in E. coli commensal or pathogenic strains can be transferredto clinically important bacteria in human health through the food chain. In addition to the acquired resistance, the formation of biofilm gives the strains tolerance to antibiotics, which contributes to the recurrence of infections. The objective of this work is to determine the susceptibility to antibiotics of E. coli strains from laying hens. One hundred and eighty three sewage exudates were collected from four productive units in Mayabeque province. Eighty three isolates such as E. coli were identified. The susceptibility in vitro to ten antibiotics was determined by the Agar Diffusion Method and the ability to form biofilm by the Plate Adhesion Method. The Minimum Inhibitory Concentration for the planktonic growth (MICP) and for the inhibition of biofilm formation (MICB) was determined.The highest percentages of resistance by the disc diffusion test were to Oxytetracycline, Norfloxacin, Ticarcillin/Clavulanic Acid, Cefoxitin and Ceftriazone; and the lowest percentages to Gentamicin, Colistin and Nitrofurantoin. Of the total of E. coli isolates, only 14 % formed biofilm in vitro. MICB was higher than MICP, which shows that biofilm formation contributes to tolerance to antibiotics.
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References
Szmolka A, Nagy B. Multidrug resistant commensal Escherichia coli in animals and its impact for public health. Front Microbiol. 2013;4(258):1-13. doi: 10.3389/fmicb.2013.00258
Chattopadhyay MK. Use of antibiotics as feed additives: a burning question. Front Microbiol. 2014;5(334):1-3. doi: 10.3389/fmicb.2014.00334
Munita J, Arias C. Mechanisms of Antibiotic Resistance. En: Kudva I, Cornick N, Plummer P, et al. (Ed). Virulence Mechanisms of Bacterial Pathogens, 5ta Ed. Washington DC: ASM Press. 2016. 481-511. doi: 10.1128/microbiolspec.VMBF-0016-2015.
Klinger-Strobel M, Lautenschläger C, Fischer D, Mainz JG, Bruns T, Tuchscherr L, et al. Aspects of pulmonary drug delivery strategies for infections in cystic fibrosis where do we stand? Expert Opin Drug Deliv. 2015;12:1351-1374.
Li J, Xie S, Ahmed S, Wang F, Gu Y, Zhang C, et al. Antimicrobial Activity and Resistance: Influencing Factors. Front Pharmacol. 2017;8(364):1-11. doi: 10.3389/fphar.2017.00364
Hall CW, Mah T-F. Molecular mechanisms of biofilm-based antibiotic resistance and tolerance in pathogenic bacteria. FEMS Microbiol Rev. 2017;41(3):276-301.
Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: A review. J Pharm Anal. 2016;6(2):71-79.
Ciofu O, Rojo-Molinero E, Macià MD, Oliver A. Antibiotic treatment of biofilm infections. APMIS. 2017;125(4):304-319.
Eckburg PB, Bik EM, Bernstein CN, Purdom E, Dethlefsen L, Sargent M, et al. Diversity of the human intestinal microbial flora. Science. 2005;308:1635–1638.
Landman W, van Eck J. The incidence and economic impact of the Escherichia coli peritonitis syndrome in Dutch poultry farming. Avian Pathol. 2015;44(5):370-378.
Tamayo M. La colibacilosis aviar y su repercusión en la avicultura. XXII Congreso Centroamericano y del Caribe en Avicultura en Panamá. 2012. [citado en Junio 2014]. Disponible en Internet: http://www.elsitioavicola.com
Smet A, Martel A, Persoons D, Dewulf J, Heyndrickx M, Catry B, et al. Diversity of extended spectrum beta-lactamases and class C beta-lactamases among cloacal Escherichia coli isolates in Belgian broiler farms. Antimicrob Agents Chemother. 2008;52:1238-1243.
Harisberger M, Gobeli S, Hoop R, Dewulf J, Perreten V, Regula G. Antimicrobial Resistance in Swiss Laying Hens, Prevalence and Risk Factors. Zoonoses Public Health. 2011;58(6):377-387.
Olson ME, Ceri H, Morck DW, Buret AG, Read RR. Biofilm bacteria: formation and comparative susceptibility to antibiotics. Can J Vet Res. 2002;66:86-92.
Instituto de Medicina Veterinaria. Listado Oficial de Productos Veterinarios Registrados. Cuarentena e inocuidad de los alimentos. Grupo de registro de productos de uso veterinario. 2014:1-123.
Van Hoorebeke S, Van Immerseel F, Berge AC, Persoons D, Schulz J, Hartung J, et al. Antimicrobial resistance of Escherichia coli and Enterococcus faecalis in housed laying-hen flocks in Europe. Epidemiol Infect. 2010;139(10):1610-1620.
Mihaiu L, Lapusan A, Tanasuica R, Sobolu R, Mihaiu R, Oniga O, et al.First report on the prevalence and antimicrobial susceptibility of Salmonella spp. isolates in retail meat products in Romania. J Infect Dev Ctries. 2014;8:50-58.
Mosquito S, Ruiz J, Bauer JL, Ochoa TJ. Mecanismos moleculares de resistencia antibiótica en Escherichia coli asociadas a diarrea. Rev Peru Med Exp Salud Publica. 2011;28(4):648-656.
Código Sanitario para los Animales Terrestres. Capítulo 6.7. -OIE Armonización de los programas nacionales de vigilancia y seguimiento de la resistencia a los agentes antimicrobianos. 2013. Disponible en Internet en: http://www.oie.int/doc/ged/D12823.PDF
Chotar M, Vidova B, Godany A. Development of specific and rapid detection of bacterial pathogens in dairy products by PCR. Folia Microbiol. 2006;51: 639-646.
Naves P, del Prado G, Huelves L, Gracia M, Ruiz V, Blanco J, et al.Measurement of biofilm formation by clinical isolates of Escherichia coli is method-dependent. J Appl Microbiol. 2008;105:585-590.
Christensen GD, Simpson WA, Younger JA, Baddour LM, Barrett FF, Melton DM, et al. Adherence of cogulase negative Staphylococci to plastic tissue cultures: a quantitative model for the adherence of staphylococci to medical devices. J Clin Microbiol. 1985;22(6):996-1006.
Instituto de Estándares Clínicos y de Laboratorios. Performance standards for antimicrobial susceptibility testing: 23th informational supplement, M100-S23. CLSI, Wayne, PA. 2013.
Comité Europeo para Pruebas de Susceptibilidad Antimicrobiana. Breakpoint tables for interpretation of MICs and zone diameters. Version 4.0. 2014 [citado Junio 2014].
Di Rienzo JA, Casanoves F, Balzarini MG, Gonzalez L, Tablada M, Robledo CW. InfoStat versión 2014. Grupo InfoStat, FCA, Universidad Nacional de Córdoba, Argentina.
Blanco J, Blanco M, Blanco JE, Mora A, Alonso MP, González EA, et al. Enterobacterias: características generales. Género Escherichia. En: Vadillo S, Piriz S, Mateos E. (Ed). Manual de Microbiología Veterinaria. España:McGraw-Hill Interamericana. 2002:301-325.
Vandekerchove D, Herdt PD, Laevens H, Butaye P, Meulemans G, Pasmans F. Significance of interactions between Escherichia coli and respiratory pathogens inlayer hen flocks suffering from colibacillosis-associated mortality. Avian Pathol. 2004;33(3):298-302.
Stordeura P, Marlier D, Blanco J, Oswald E, Biet F, Dho-Moulin M, et al. Examination of Escherichia coli from poultry for selected adhesin genes important in disease caused by mammalian pathogenic E. coli. Vet Microbiol. 2002;84:231-241.
Barnes H, Nolan L, Vaillancourt JP. Colibacillosis. En: Saif Y. (Ed). Diseases of poultry. 12ª Ed. Iowa: Blackwell Publishing. 2008:691-732.
Cheng G, Dai M, Ahmed S, Hao H, Wang X, Yuan Z. Antimicrobial drugs in fighting against antimicrobial resistance. Front Microbiol. 2016;7(470):1-11. doi: 10.3389/fmicb.2016.00470
Wright GD. Antibiotic resistance in the environment: a link to the clinic? Curr Opin Microbiol. 2010;13:589–594.
Yap PS, Yiap BC, Ping C, Lim SH. Essential oils, a new horizon in combating bacterial antibiotic resistance. Open Microbiol J. 2014;8:6-14.
Mihaiu L, Lapusan A, Tanasuica R, Sobolu R, Mihaiu R, Oniga O, et al.First report on the prevalence and antimicrobial susceptibility of Salmonella spp. isolates in retail meat products in Romania. J Infect Dev Ctries. 2014;8:50-58.
Torres C, Zarazaga M. BLEE en animales y su importancia en la transmisión a humanos. Enferm Infecc Microbiol Clin. 2007;25:29-37
Johnson TJ, Logue CM, Johnson JR, Kuskowski MA, Sherwood JS, Barnes HJ, et al. Associations between multidrug resistance, plasmid content, and virulence potential among extraintestinal pathogenic and commensal Escherichia coli from humans and poultry. Foodborne Pathog Dis. 2012;9:37-46.
Alcaine S, Molla L, Nugen S, Kruse H. Results of a pilot antibiotic resistance survey of Albanian poultry farms. J Glob Antimicrob Resist. 2016;4:60-64.
Rugumisa BT, Call DR, Mwanyika GO, Mrutu RI, Luanda CM, Lyimo BM, et al. Prevalence of Antibiotic-Resistant Fecal Escherichia coli Isolates from Penned Broiler and Scavenging Local Chickens in Arusha, Tanzania. J Food Prot. 2016;79(8):1424-1429.
Eager H, Swan G, van Vuuren M. A survey of antimicrobial usage in animals in South Africa with specific reference to food animals. J S Afr Vet Assoc. 2015;83: 16.
Yang H, Chen S, White DG, Zhao S, McDermott P, Walker R, et al. Characterization of Multiple-Antimicrobial-Resistant Escherichia coli Isolates from Diseased Chickens and Swine in China. J Clin Microbiol. 2004; 42(8):3483–3489.
Wang Y, Yi L, Wang Y, Wang Y, Cai Y, Zhao W, et al. Isolation, phylogenetic group, drug resistance, biofilm formation, and adherence genes of Escherichia coli from poultry in central China. Poult Sci. 2016;95(12):2895-2901.
Rodríguez-Martínez JM, Cano ME, Calvo J, Hernández P, Martinez A, Martinez L. Plasmid-mediated quinolone resistance. En: Krcmery V. (Ed.). Resistance. London: Future Medicine Ltd; 2013.107-121.
Hopkins KL, Davies, RH, Threlfall EJ. Mechanisms of quinolone resistance in Escherichia coli and Salmonella: recent developments. Int J Antimicrob Agents. 2005;25(5):358-373.
Abreu R, Castro-Hernández B, Madueño A, Espigares-Rodríguez E, Moreno-Roldán E, Moreno P. Cepas de E. coli productoras de betalactamasas (BLEE) aisladas en pollos en Tenerife (España). Hig Sanid Ambient. 2013;13(4):1091-1096.
Li XS, Wang GQ, Du XD, Cui BA, Zhang SM, Shen JZ. Antimicrobial susceptibility and molecular detection of chloramphenicol and florfenicol resistance among Escherichia coli isolates from diseased chickens. J Vet Sci. 2007;8(3):243-247.
Gilbert M. Detección y caracterización de aislados de Escherichia coli de origen clínico y fecal en gallinas ponedoras [tesis]. Madrid: Universidad Complutense de Madrid. 2009. Disponible en Internet en: http://eprints.ucm.es/10514/
Amaral A, Zakia Z, Bouzoubaa K. Antibioresistance of Escherichia coli strains isolated in Morocco from chickens with colibacilosis. Vet Microbiol. 1995;43:325-330.
Geornaras L, Hastings JW, von Holy A. Genotypic analysis of Escherichia coli from strains poultry carcasses and their susceptibilities to antimicrobial agents. Appl Envirom Microbiol. 2001;67(4):1940-1944.
Liu YY, Wang Y, Walsh TR, Yi LX, Zhang R, Spencer J, et al. Emergence of plasmid -mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study. Lancet Infect Dis. 2016;16(2):161-168. doi: 10.1016/S1473-3099(15)00424-7
Organización Panamericana de la Salud (OPS/OMS). Alerta epidemiológica: Enterobacterias con resistencia transferible a colistina. Implicaciones para la salud pública en las Américas. 2016. [citado 7 de diciembre 2016]. Disponible en internet en:http://antimicrobianos.com.ar/ATB/wp-content/uploads/2016/06/Enterobacterias-con-resistencia-transferible-a-colistina.pdf
Agencia Europea de Medicamentos. Use of colistin-containing products within the European Union: development of resistance in animals and possible impact on human and animal health. 2013. [citado 7 de diciembre 2016]. Disponible en internet en:http://www.ema.europa.eu/docs/en_GB/document_library/Report/2013/07/WC500146813.pdf
Gupta K, Hooton TM, Roberts PL, Stamm WE. Short-course nitrofurantoin for the treatment of acute uncomplicated cystitis in women. Arch Intern Med. 2007;167(20):2207-2212.
Alonso CA, Zarazaga M, Ben Sallem R, Jouini A, Ben Slama K, Torres C. Antibiotic resistance in Escherichia coli in husbandry animals: the African perspective. Lett Appl Microbiol. 2017;64(5):318-334.
Reisner A, Krogfelt KA, Klein BM, Zechner EL, Molin S. In vivo biofilm formation of commensal and pathogenic Escherichia coli strains: Impact of environmental and genetic factors. J Bacteriol. 2006;188:3572-3581.
Pratt LA, Kolter R. Genetic analysis of Escherichia coli biofilm formation: roles of flagella, motility, chemotaxis and type I pili. Mol Microbiol. 1998;30(2):285-293.
Yonezawa H, Osaki T, Kamiya S. Biofilm formation by Helicobacter pylori and its involvement for antibiotic resistance. Biomed Res Int. 2015;2015:914791:1-9. doi: 10.1155/2015/914791
Costerton JW, Stewart PS, Greenberg EP. Bacterial biofilms: a common cause of persistent infections. Science. 1999;284:318–322.
Penesyan A, Gillings M, Paulsen IT. Antibiotic discovery: combatting bacterial resistance in cells and in biofilm communities. Molecules. 2015;20:5286–5298.
Ramadan HH. Chronic rhinosinusitis and bacterial biofilms. Curr Opin Otolaryngol Head Neck Surg. 2006;14(3):183-186.
Soto SM. Role of efflux pumps in the antibiotic resistance of bacteria embedded in a biofilm. Virulence. 2013;4(3):223-229.