Nuevas tendencias en el diagnóstico de enfermedades virales en los animales

Carmen Laura Perera, Ana María Acevedo

Resumen

El diagnóstico y control de las enfermedades virales de importancia económica ha progresado de forma notable en la última década, gracias a la aplicación de las tecnologías de reacción en cadena de la polimerasa (PCR) y a las diferentes aplicaciones de la misma, como son la reacción de PCR basadas en la detección de fluorescencia en tiempo real (rPCR), la amplificación isotérmica mediada por bucle (LAMP) y las técnicas basadas en la secuenciación de ácidos nucleicos. Estos enfoques permiten la amplificación, la cuantificación simultánea y el análisis de secuencias de ácidos nucleicos, donde se combinan rapidez con elevadas sensibilidad y especificidad; sus beneficios con relación a los ensayos de PCR convencional o de punto final incluyen, además de un elevado rango dinámico, reducido riesgo de contaminación cruzada, capacidad para ser escalados y cuantificación precisa de la diana, lo que permite la determinación de la carga viral.

Palabras clave

diagnóstico molecular; PCR; rPCR; secuenciación; LAMP

Texto completo:

PDF HTML XML-JATS EPUB

Referencias

-Herrero U. Capítulo 6. Estrategias generales para el diagnóstico en virología. En: Procedimientos en Virología Médica. 1. Ed. San José. C.R. Editorial de la Universidad de Costa Rica. 2004. Pp. 80-93.

-Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, et al. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science. 1985;230(4732):1350-4.

-Hoffmann B, Beer M, Reid SM, Mertens P, Oura CA, van Rijn PA, et al. A review of RT-PCR technologies used in veterinary virology and disease control: sensitive and specific diagnosis of five livestock diseases notifiable to the World Organisation for Animal Health. Vet Microbiol. 2009; 39:1-23.

-Belák S. Molecular diagnosis of viral diseases, present trends and future aspects A view from the OIE Collaborating Centre for the Application of Polymerase Chain Reaction Methods for Diagnosis of Viral Diseases in Veterinary Medicine. Vaccine. 2007;25:5444-5452.

-Skerra A. Phosphorothioate primers improve the amplification of DNA sequences by DNA polymerases with proofreading activity. Nucleic Acids Res. 1992;20:3551-3554.

-Wagar EA. Direct hybridization and amplification applications for the diagnosis of infectious diseases. J Clin Lab Anal.1996;10:312-325.

-Mittermeier RA. Conservation International and biodiversity conservation. Nature. 2000;405:255.

-Qian K. Detection of HCV RNA in serum by reverse transcription polymerase chain reaction (RT-PCR). Methods Mol Med. 1999;19:47-53.

-Dynon K, Varrasso A, Ficorilli N. Identification of equine herpesvirus 3 (equine coital exanthema virus), equine gammaherpesviruses 2 and 5, equine adenoviruses 1 and 2, equine arteritis virus and equine rhinitis A virus by polymerase chain reaction. Aust Vet J. 2001;79:695-702.

-Erlich HA, Gelfand D, Sninsky JJ. Recent advances in the polymerase chain reaction. Science. 1991;252:1643-1651.

-Zhang L, Pan Z, Geng S. Sensitive, semi-nested RT-PCR amplification of fusion gene sequences for the rapid detection and differentiation of Newcastle disease virus. Res Vet Sci. 2010;89:282-289.

-Elnifro EM, Ashshi AM, Cooper RJ, Klapper PE. Multiplex PCR: optimization and application in diagnostic virology. Clin Microbiol Rev. 2000;13:559-570.

-Chamberlain JS, Gibbs RA, Ranier JE. Deletion screening of the Duchenne muscular dystrophy locus via multiplex DNA amplification. Nucleic Acids Res. 1988;16:11141-11156.

-Mishra B, Sharma M, Pujhari SK. Clinical applicability of single-tube multiplex reverse-transcriptase PCR in dengue virus diagnosis and serotyping. J Clin Lab Anal. 2011;25:76-78.

-Mihaly I, Kolozsi T, Liptai Z. Experience with multiplex nested PCR and fluorescent antibody tests in the diagnosis of acute central nervous system infections with herpes simplex virus type 1 and 2. Orv Hetil. 2010;151:1896-1903.

-Beck ET, Henrickson KJ. Molecular diagnosis of respiratory viruses. Future Microbiol. 2010;5:901-916.

-Valasek MA, Repa JJ. The power of real-time PCR. Advances in Physiology Education. 2005;29: 151-159.

- Zipper H, Brunner H, Bernhagen J, Vitzthum F. Investigations on DNA intercalation and surface binding by SYBR green I, its structure determination and methodological implications. Nucleic Acids Res. 2004;32:103.

- Livak KJ, Flood SJ, Marmaro J, Giusti W, Deetz K. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. PCR Methods Appl. 1995;4:357-362.

-Tyagi S, Kramer FR. Molecular beacons: probes that fluoresce upon hybridization. Nat Biotechnol. 1996;14:303-308.

-Acevedo AM, Perera CL, Vega A, Ríos L, Coronado L, Relova D, et al. A duplex SYBR Green I-based real-time RT-PCR assay for the simultaneous detection and differentiation of Massachusetts and non-Massachusetts serotypes of infectious bronchitis virus. Mol Cell Probes. 2013;27(5-6):184-92.

-Tam S, Clavijo A, Engelhard EK, Stanley T, Alfonso C, Eric KE, et al. Fluorescence-based multiplex real-time RT-PCR arrays for the detection and serotype determination of foot-and-mouth disease virus. J Virol Methods. 2009;161:183-191.

-Huang YL, Pang VF, Pan CH, Chen TH, Jong MH, Huang TS, et al. Development of a reverse transcription multiplex real-time PCR for the detection and genotyping of classical swine fever virus. J Virol Methods. 2009;160:111-118.

-Haines FJ, Hofmann MA, King DP, Drew TW, Crooke HR Development and Validation of a Multiplex, Real-Time RT PCR Assay for the Simultaneous Detection of Classical and African Swine Fever Viruses. PLoS ONE. 2013;8(7): e71019. doi:10.1371/journal.pone.0071019.

-Hymas WC, Mills A, Ferguson S. Development of a multiplex real-time RTPCR assay for detection of influenza A, influenza B, RSV and typing of the 2009-H1N1 influenza virus. J Virol Methods. 2010;167:113-118.

-Shisong F, Jianxiong L, Xiaowen C. Simultaneous detection of influenza virus type B and influenza A virus subtypes H1N1, H3N2, and H5N1 using multiplex real-time RT-PCR. Appl Microbiol Biotechnol. 2011;90:1463-1470.

-Chen Y, Cui D, Zheng S. Simultaneous detection of influenza A, influenza B, and respiratory syncytial viruses and subtyping of influenza A H3N2 virus and H1N1 (2009) virus by multiplex real-time PCR. J Clin Microbiol. 2011;49:1653-1656.

-Beck ET, Jurgens LA, Kehl SC. Development of a rapid automated influenza A, influenza B, and respiratory syncytial virus A/B multiplex real-time RTPCR assay and its use during the 2009 H1N1 swine-origin influenza virus epidemic in Milwaukee, Wisconsin. J Mol Diagn. 2010;12:74-81.

-Pérez LJ, Perera CL, Frías MT, Núñez JI, Ganges L, Díaz de Arce H. A multiple SYBR Green I-based real-time PCR system for the simultaneous detection of porcine circovirus type 2, porcine parvovirus, pseudorabies virus and Torque teno sus virus 1 and 2 in pigs. J Virol Methods. 2012;179:233-241.

-Rodríguez-Tarduchy G. ¿Hablamos de gen o más? 2007. http://www.editorialhelice.es/serie-tangente/hablamos-de-gen-o-mas.html

- Genome News Network. 2004. (http://www.genomenewsnetwork.org

-Venter JC. The sequence of the human genome. Science. 2001;291(5507):1304-1351.

- Metzker ML. Sequencing technologies the next generation. Nature Reviews Genetics. 2010;11:31-46.

- Deyde VM, Nguyen T, Bright RA, Balish A, Shu B, Lindstrom S, et al. Detection of molecular markers of antiviral resistance in influenza A (H5N1) viruses using a pyrosequencing method. Antimicrob Agents Chemother. 2009;53(3):1039-1047.

- Bishop-Lilly A. Arbovirus Detection in Insect Vectors by Rapid, High- Throughput Pyrosequencing. PLoS Negl Trop Dis. 2010;4(11):878.

- Lin BC, Malanoski AP, Wang Z, Blaney KM, Long NC, Meador CE, et al. Universal detection and identification of avian influenza virus by use of resequencing microarrays. J Clin Microbiol. 2009;47(4):988-993.

- Huang Y, Duffy S, Hong Y, Norman S, Ghosh M, He J, et al. Multiplex assay for simultaneously typing and subtyping influenza viruses by use of an electronic microarray. J Clin Microbiol. 2009;47(2):390-396.

- Njiru ZK, Mikosza AS, Armstrong T, Enyaru JC, Ndung'u JM, Thompson AR. Loop-Mediated Isothermal Amplification (LAMP) Method for Rapid Detection of Trypanosoma brucei rhodesiense. PLoS Negl Trop Dis. 2008;2(2):e147. doi:10.1371/journal.pntd.0000147.

- Ito M. Rapid detection and typing of influenza A and B by loop-mediated isothermal amplification: comparison with immunochromatography and virus isolation. J Virol Methods. 2006;135(2):272-275.

Enlaces refback

  • No hay ningún enlace refback.