Evaluation of physical-chemical and microbiological parameters of a rennet from pig

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Published: Apr 1, 2019
Keywords:
rennet, drying method, physical-chemical parameters, microbiological parameters

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Marisney Martinez-Alvarez
Centro Nacional de Sanidad Agropecuaria
Dianys Remón-Díaz
Centro Nacional de Sanidad Agropecuaria
Aldo Hernández Monzón
Universidad de La Habana
Yamilka Riverón-Alemán
Centro Nacional de Sanidad Agropecuaria
Ailin Martínez-Vasallo
Centro Nacional de Sanidad Agropecuaria

Abstract

The physical-chemical and microbiological parameters of a rennet from pig stomachs were evaluated. The stomachs were washed, salted and dehydrated naturally. The moisture content of stomachs was determined on alternate days. The pH, rennet strength, microorganism counts at 30°C, total coliforms, Escherichia coli, Enterobacteria, Staphylococcus coagulase positive, fungi, and yeasts, were evaluated. No significant differences were observed on the first day through the analysis of variance when evaluating the behavior of the stomachs, in terms of water content per gram of dry matter by the two drying methods with the addition of sodium chloride. The pH values were in the range of 4.44 to 4.90 and those of rennet strength between 1: 840 to 1: 4706 for the 15 runs evaluated. The mean microorganism count at 30°C was 2.05 ± 0.76 log cfu / mL. The counts of total coliforms, enterobacteria, fungi, and yeast were less than 10 cfu / ml. The presence of positive coagulase Staphylococcus or Escherichia coli was not detected. Obtaining the milk coagulant from pig stomach provides an alternative to improve the production process of artisan fresh cheese, when it is verified that the physical-chemical and microbiological parameters are in correspondence with what established for rennet.

Article Details

How to Cite
1.
Martinez-Alvarez M, Remón-Díaz D, Hernández Monzón A, Riverón-Alemán Y, Martínez-Vasallo A. Evaluation of physical-chemical and microbiological parameters of a rennet from pig. Rev. Salud Anim. [Internet]. 2019 Apr. 1 [cited 2024 Nov. 21];41(1). Available from: https://revistas.censa.edu.cu/index.php/RSA/article/view/1005
Issue
Vol. 41 No. 1 (2019): January-April
Section
ARTÍCULOS ORIGINALES
National Center for Animal and Plant Health (CENSA)

References

González M. Tecnología para la elaboración de queso blanco, amarillo y yogurt. Ciencia y Tecnologías de Alimentos Secretaria Nacional de Ciencias, Tecnología e Innovación Veraguas, Panamá. 2002.

De Jong L. Nuevo cuajo estándar. Alimentación, equipos y tecnología. 1990;9(3):123-131.

Acevedo RM, Vasek OF, Fusco AJV. Optimización de un secadero de cuajares para la producción de agente coagulante correntino. Universidad Nacional del nordeste, Comunicaciones Científicas y Tecnológicas, Argentina. 2006.

Coronel GJ, Fusco AJV, Olga M, López MB. Quesos artesanales de Corrientes: Caracterización del agente coagulante. Comunicaciones Científicas y Tecnológicas, Universidad Nacional del Nordeste. 2014;8(8):77-80.

Martínez A, Ribot A, Villoch A, Montes de Oca N, Remón D, Ponce P. Calidad e inocuidad de la leche cruda en las condiciones actuales de Cuba. Rev Salud Anim. 2017;39(1):51-61.

Bonafede M, Aimar B, Nieto I, Picotti J, Molina S. Determinación de parámetros fisicoquímicos y microbiológicos para la elaboración y conservación artesanal de cuajo fermento de cabrito. 2016. Consultado: 25 marzo 2018. Disponible en: https://www.inti.gob.ar/lacteos/pdf/cuajoCabrito.pdf.

Moschopoulou E, Kandarakisa I, Anifantakis E. Characteristics of Lamb and kid artisanal liquid rennet used for traditional feta cheese manufacture. Small Ruminant Res. 2006;72:237-241.

NC 275. Carne y productos cárnicos. Determinación del contenido de humedad. Método rápido. Norma Cubana (NC). La Habana. 2003. pp 7.

Dardas R, inventor; Google Patents, assignee. Preservation of bovine stomachs for rennet extraction. US3766015A. United States. 1973.

ISO 10523. Water quality. Determination of pH. 2008. 13 p

Chazarra S, Sidrach L, López D, Rodríguez J. Characterization of the milk-clotting properties of extracts from artichoke (Cynara scolymus L.) flowers. Int. Dairy J. 2007;17:1393-1400.

Spreer E. Lactología industrial: leche, preparación y elaboración, máquinas, instalaciones y aparatos, productos lácteos. Zaragoza. España. Acribia, DL, 2 ed 1991. 626p. ISBN: 978-84-200-0715-1.

ISO 4833-1. Microbiology of the food chain-Horizontal method for the enumeration of microorganisms-Part 1: Colony count at 30 degrees C by the pour plate technique. 2013. 9 p.

Norma ISO 4832. Microbiology of food and animal feeding stuffs-Horizontal method for the enumeration of coliforms. Colony-count technique. 2006. 6p.

Norma ISO 16649-2. Microbiology of food and animal feeding stuffs-Horizontal method for the enumeration of a-glucuronidase-positive Escherichia coli-Part 2: Colony-count technique at 44°C using 5-bromo-4-chloro-3-indolyl a-D-glucuronide. 2001. 8p.

ISO 21528-2. Microbiology of the food chain - Horizontal method for the detection and enumeration of Enterobacteriaceae Part 2: Colony-count technique. 2017. 15p.

ISO 6888-1. Microbiology of food and animal feeding stuffs-Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species). Part 1: Technique using Agar Baird Parker medium. 2003. 6p.

ISO 21527-2. Microbiology of food and animal feeding stuffs-Horizontal method for the enumeration of yeasts and moulds-Part 2: Colony count technique in products with water activity less than or equal to 0,95. 2008. 9p.

Di rienzo JA, Balzarini MG, Gonzalez L, Tablada M, Robledo CW. InfoStat versión 2.0. 2010. Grupo InfoStat, FCA. Universidad Nacional de Córdoba, Argentina. 2010.

Sheehan JJ. How does NaCl affect the microbiology of cheese? In: P.L. McSweeney, editor, Cheese problems solved. Woodhead Publishing Limited, and CRC Press LLC, Cambridge, GBR. 2007:96-97.

Cruz AG, Faria JA, Pollonio MA, Bolini HM, Celeghini RM, Granato D. Cheeses with reduced sodium content: Effects on functionality, public health benefits and sensory properties. Trends in Food Science & Technology. 2011;22(6):276-291.

Gorreta L. Coahlo e coagulantes. Revista do Instituto do Lacticinios Candido Tostes. 2000; 222 (47): 19-24.

Dobler J, Espinosa E, Hernández PA, López LX, Márquez O. Extracto coagulante de leche proveniente del estómago de conejo (Oryctolagus cuniculus sp.). Agrociencia. 2016; 5 (50): 583-593.

Mahler HR, Cordes EH. Química Biológica. 4 ed. Barcelona. Omega. 1990. 893p.

Moschopoulou E. Characteristics of rennet and other enzymes from small ruminants used in cheese production. Small Ruminant Research. 2011;101:188-195.

Córdova JS, Paitan E. Determinación de parámetros para obtención y conservación de cuajo de bovino adulto. Ciencia e Investigación. 2013;16(1):9-11.

Palladino PM, Rodríguez HR, Molina RA, Ortigoza G, Moreno K, Chavez M. Inocuidad microbiológica de cuajos utilizados en la elaboración de quesos de cabra artesanales en el valle de amblayo, provincia de Salta, Argentina. Jornada de Actualización Técnica en el Instituto de Tecnología de Alimentos, 23 de Septiembre de 2012. Hurlingham, Buenos Aires AR. 2012.

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