In vitro evaluation of the probiotic potential of Lactobacillus acidophilus SS80 and Streptococcus thermophilus SS77

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Published: Apr 1, 2019
Keywords:
zootechnical additive, lactic acid bacteria, Lactobacillus acidophilus SS80, probiotic, Streptococcus thermophilus SS77

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Juan Emilio Hernández-García
Universidad de Sancti Spíritus. “José Martí Pérez”
Laureano Sebastián-Frizzo
Instituto de Ciencias Veterinarias del Litoral
Juan Carlos Rodríguez-Fernández
Universidad de Sancti Spíritus. “José Martí Pérez”
Gregory Valdez-Paneca
Universidad de Sancti Spíritus. “José Martí Pérez”
María Virginia-Zbrun
Instituto de Ciencias Veterinarias del Litoral
Ibrahin Calero-Herrera
Universidad de Sancti Spíritus. “José Martí Pérez”

Abstract

The objective of this work was to evaluate in vitro the probiotic properties of Lactobacillus acidophilus SS80 and Streptococcus thermophilus SS77 strains for animal use. Strains were characterized by biochemical tests, hemolysis, resistance to different pH, bile salt concentrations, microbial antagonism test against five animal pathogens, and auto-aggregation, co-aggregation and hydrophobicity tests. The results showed that both strains produce hydrogen peroxide, lipase and lecithinase. S. thermophilus SS77 was positive to casein proteolysis; however, the strains did not produce biogenic amines from the amino acids Lysine, Tyrosine and Tryptophan. In addition, S. thermophilus SS77 used alanine from 36 h on, whereas L. acidophilus SS80 used histidine from 72 h on. Both strains were negative to the hemolysis test and differed significantly in the pH values reached in skimmed milk powder after 2 h. They grew at pH 6.5 and 5.5 and resisted the simulated gastric juice for 3 h, with survival rates above 98 %. The strain of L. acidophilus SS80 grew only at 0.3 % bile (% w / v), while S. thermophilus SS77 did it at 0.3 %, 0 % and 1 % (% w / v). Growth inhibition occurred on all pathogenic strains evaluated, being Lactobacillus acidophilus SS80 more effective. Both strains were positive to the auto-aggregation, co-aggregation and hydrophobicity tests with values higher than 48 %. It is concluded that both strains have potential to be used as probiotic additives in animal feeding.

Article Details

How to Cite
1.
Hernández-García JE, Sebastián-Frizzo L, Rodríguez-Fernández JC, Valdez-Paneca G, Virginia-Zbrun M, Calero-Herrera I. In vitro evaluation of the probiotic potential of Lactobacillus acidophilus SS80 and Streptococcus thermophilus SS77. Rev. Salud Anim. [Internet]. 2019 Apr. 1 [cited 2024 Nov. 21];41(1). Available from: https://revistas.censa.edu.cu/index.php/RSA/article/view/1008
Issue
Vol. 41 No. 1 (2019): January-April
Section
ARTÍCULOS ORIGINALES
National Center for Animal and Plant Health (CENSA)

References

Kleerebezem M, Binda S, Bron PA, Gross G, Hill C, van Hylckama Vlieg JE, et al. Understanding mode of action can drive the translational pipeline towards more reliable health benefits for probiotics. Current Opinion in Biotechnology. 2019;56:55-60.

Di Gioia D, Biavati B. Probiotics and prebiotics in Animal Health and Food Safety. 2018(DOI.org/10.1007/978-3-319-71950-4_9):219-45.

Hernández JE, Calero I, Reyes J, Rodríguez JC. Evaluation of the practical clinical effect of feed supplements with lactic bacteria in young calves. Rev NEWSLETTER. 2001;3.

Foko KEM, Zambou NF, Kaktcham PM, Wang RY, Zhu T, Yin L. Screening and Characterization of Lactobacillus sp. from the Water of Cassava's Fermentation for Selection as Probiotics. Food Biotechnol. 2018;32(1):15-34.

Hill C, Guarner F, Reid G, Gibson GR, Merenstein DJ, Pot B, et al. Expert consensus document. The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nature Reviews Gastroent Hepat. 2014;11(8):506-514.

Geraldi MV, Tulini FL, Souza VM, et al. Development of Yoghurt with Juçara Pulp (Euterpe edulis M.) and the Probiotic Lactobacillus acidophilus La5 . Probiotics Antimicro Prot. 2018;10(1):71-76.

Miranda-Yuquilema JE, Marin-Cárdenas A, González-Pérez M. The bioproductive behavior of breeding sows and their offspring fed with probiotic additive. Rev Ciencias Agrícolas. 2018;35(1):69-81.

Khare A, Thorat G, Bhimte A, Yadav V. Mechanism of action of prebiotic and probiotic. J Entomol Zoology Studies. 2018;6(4):51-3.

Plaza-Diaz J, Ruiz-Ojeda FJ, Gil-Campos M, Gil A. Mechanisms of action of probiotics. Advances in Nutrition. 2019;10(suppl_1):S49-S66.

Cossio DS, Hernández YG, Mendoza JD. Development of probiotics for animal production. Experiences in Cuba Desarrollo de probióticos destinados a la producción animal: experiencias en Cuba. Rev Ciencias Agrícolas. 2018;52(4):1.

Vega-Cañizares. Eficacia de un probiótico sobre Escherichia coli K88 en cerdos. Rev Salud Anim. 2018;40(1).

Sánchez L, Omura M, Lucas A, Pérez T, Llanes M, Ferreira C. Cepas de Lactobacillus spp. con capacidades probióticas aisladas del tracto intestinal de terneros neonates. Rev Salud Anim. 2015;37(2):94-104.

McLean NW, Rosenstein IJ. Characterisation and selection of a Lactobacillus species to re-colonise the vagina of women with recurrent bacterial vaginosis. J Medical Microbiology. 2000;49:543-52.

Rosenstein IJ, Fontaine EA, Morgan DJ, Sheehan M, Lamont RF, Taylor-Robinson D. Relationship between hydrogen peroxide-producing strains of lactobacilli and vaginosis-associated bacterial species in pregnant women. Clinical Microbiol Infec Dis. 1997;16:517-522.

Mete A, Cosansu S, Demirkol O, Ayhan K. Amino acid decarboxylase activities and biogenic amine formation abilities of lactic acid bacteria isolated from shalgam. Int J Food Prop. 2017;20(171-178).

Harrigan WF. Laboratory Methods in Food Microbiology. Press. A, editor.1998.

MacFaddin JF. Pruebas bioquímicas para la identificación de bacterias de importancia clínica Panamericana EeBAEM, editor. 2003 .

Balamurugan R, Chandragunasekaran AS, Chellappan G, Rajaram K, Ramamoorthi G, Ramakrishna BS. Probiotic potential of lactic acid bacteria present in home made curd in southern India. Indian J Med Res. 2014;140(3):345.

Pérez J, Rocha E, Uzcategui D, Aranguren Y, Machado E. Aislamiento, selección y caracterización de Lactobacillus genus aisladas del líquido ruminal vacuno en la zona sur del lago, Venezuela. Rev Colombiana Ciencia Animal. 2015;7(2):165-170.

Bao Y, Zhang Y, Liu Y, Wang S, Dong X, Wang Y, et al.Screening of potential probiotic properties of Lactobacillus fermentum isolated from traditional dairy products. Food Control. 2010;21(5):695-701.

Uriot O, Denis S, Junjua M, Roussel Y, Dary-Mourot A, Blanquet-Diot S. Streptococcus thermophilus: From yogurt starter to a new promising probiotic candidate? J Functional Foods. 2017;37:74-89.

Kajal A, Ankur G, Jagriti S. Isolation and Identification of Lactobacilli Bacteria from Raw Cow Milk in Local Region of Agra. Int J Adv Res Biol Sci. 2017;4(11):98-102.

Maldonado NC, Ficoseco CA, Mansilla FI, Melián C, Hébert ME, Vignolo GM, et al. Identification, characterization and selection of autochthonous lactic acid bacteria as probiotic for feedlot cattle. Livestock Science. 2018.

Sánchez L, Tromps J. Caracterización in vitro de bacterias ácido lácticas con potencial probiótico. . Rev Salud Anim. 2014; 36(2):124-129.

Ladero V, Martín MC, Redruello B, Mayo B, Flórez AB, Fernández M, et al. Genetic and functional analysis of biogenic amine production capacity among starter and non-starter lactic acid bacteria isolated from artisanal cheeses. Eur Food Res Technol. 2015;241(377-383).

Pereira CI, Barreto-Crespo MT, San Romão MV. Evidence for proteolytic activity and biogenic amines production in Lactobacillus curvatus and Lactobacillus homohiochii. International J Food Microbiol. 2001;68:211-216.

Pircher A, Friedrich B, Paulsen P. Formation of cadaverine, histamine, putrescine and tyramine by bacteria isolated from meat, fermented sausages and cheeses. Eur Food Res Technol. 2007;226:225-231.

Tarrah A, De Castilhos J, Rossi RC, da Silva Duarte V, Ziegler DR, Corich V, et al. In vitro probiotic potential and anti-cancer activity of newly isolated folate-producing Streptococcus thermophilus strains. Front Microbiology. 2018;9.

Peres Cátia M, Hernandez-Mendoza AM, Moreira LS, Bronze MR, Vilas-Boas L, M PC. Novel isolates of lactobacilli from fermented Portuguese olive as potential probiotics. LWT - Food Sci Technol. 2014;59:234-246.

Pelaez C, Requena T. Exploiting the potential of bacteria in the cheese ecosystem. Int J Dairy Technol. 2005;15:831-844.

Aimutis WR. Bioactive properties of milk proteins with particular focus on anticariogenesis. J Nutr. 2004;134:989S-995S.

FAO/WHO. Guide lines for the evaluation of probiotics in food. . Food and Agriculture Organization of the United Nations and World Health Organization Working Group Report, 1-11; London, Ontario. 2002.

Kalui CM, Mathara JM, Kutima PM, Kiiyukia C, Wongo LE. Functional characteristics of Lactobacillus plantarum and Lactobacillus rhamnosus from ikii, a Kenyan traditional fermented maize porridge. Afr J Biotechnol. 2009;8:4363-4373.

Bauman G, Longo G. El Yogurt: Un Alimento Esencial. 2015.

Jurado-Gámez H, Martínez JA, Chaspuengal A, Calpa MF. Evaluación in vitro de la acción de Lactobacillus plantarum con características probióticas sobre Yersinia pseudotuberculosis. Biotecnología en el Sector Agropecuario y Agroindustrial. 2014;12(2):49-59.

Velázquez-López A, Covatzin-Jirón D, Toledo-Meza MD, Vela-Gutiérrez G. Fermented drink elaborated with lactic acid bacteria isolated from chiapaneco traditional pozol. CienciaUAT. 2018;13(1):165-78.

Rondón AJ, Samaniego LM, Rodríguez S, Milián G, Ranilla MJ, et al.Isolation identification and partial characterization of the probiotic properties of Lacto Bacillus spp. strains obtained from the gastrointestinal tract of broilers. Rev Cienc Tecnol Aliment. 2008;6:56.

Muñoz-Atienza E, Gómez-Sala B, Araújo C, Campanero C, Del Campo R, Hernández PE, et al.Antimicrobial activity, antibiotic susceptibility and virulence factors of lactic acid bacteria of aquatic origin intended for use as probiotics in aquaculture. BMC Microbiol. 2013;13:15-22.

Oh A, Banan-Mwine DE, Deog HO. Screening for potential probiotic bacteria from Korean fermented soybean paste: In vitro and Caenorhabditis elegans model testing. LWT - Food Sci Technol. 2018;88:132-138.

Seah YN, Siew SK, Birdie SP, Fook YC. Evaluation of probiotic potential of lactic acid bacteria isolated from traditional Malaysian fermented Bambangan (Mangifera pajang) CyTA -J Food. DOI:101080/1947633720151020342. 2015.

de Souza BMS, Borgonovi TF, Casarotti SN, et al. Lactobacillus casei and Lactobacillus fermentum Strains Isolated from Mozzarella Cheese: Probiotic Potential, Safety, Acidifying Kinetic Parameters and Viability under Gastrointestinal Tract Conditions. Probiotics & Antimicro Prot. 2018;10:1-15.

Bautista-Gallego J, Arroyo-López FN, Rantsiou K, Jiménez-Díaz R, Garrido Fernández A, CocolinL. Screening of lactic acid bacteria isolated from fermented table olives with probiotic potential. Food Res Internat. 2013;50:135-142.

Iranmanesha M, Ezzatpanah H, Mojgani N. Antibacterial activity and cholesterol assimilation of lactic acid bacteria isolated from traditional Iranian dairy products LWT - Food Sci Technol. 2014;58:355-359.

Servin LA. Antagonistic activities of lactobacilli abd bifidobacteria against microbial pathogens. FEMS Microbiology Reviews. 2004;28:405-440.

Bhuiyan R, Shill S, Islam A, Chakrabortty S. Screening of Lactobacillus spp. from raw goat milk showing probiotic activities against pathogenic bacteria. Afr J Microbiol Res. 2017;11(15):620-655.

Nader-Macias ME. Advances in the design of probiotic products for the prevention of major diseases in dairy cattle. J Ind Microbiol Biot. 2008;35(11):1387-1395.

Frizzo LS, Soto LP, Zbrun MV, Signorini ML, Bertozzi E, Sequeira Gl. Effect of lactic acid bacteria and lactose on growth performance and intestinal microbial balance of artificially reared calves Livest Sci. 2011;140:246-252.

Frizzo LS, Soto LP, Bertozzi E, Sequeira G, Marti LE, Rosmini MR. Evaluación in vitro de las capacidades probióticas microbianas orientadas al diseño de inóculos probióticos multiespecie para ser utilizados enla crianza de terneros. Revista FAVE - Ciencias Veterinarias. 2006;5:1-2

Li Q, Liu X, Dong M, Zhou J, Wang Y. Aggregation and adhesion abilities of 18 lactic acid bacteria strains isolated from traditional fermented food. Int J Agric Policy Res. 2015;3(2):84-92.

Wadstrom T, Anderson K, Sydow M, Axelsson L, Lindgren S, Gullmar B. Surface properties of lactobacilli isolated from the small intestine of pigs. J Appl Bacteriol. 1987;62:513-520

Devi SM, Archer AC, Halami PM. Screening, characterization and in vitro evaluation of probiotic properties among lactic acid bacteria through comparative analysis. Probiotics and antimicrobial proteins. 2015;7(3):181-192.

Ehrmann MA, Bauer J, Vogel RF. Characterization of lactobacilli towards their use as probiotic adjuncts in poultry. J Appl Microbiol. 2002;92:966-975.

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