Detection of microorganisms of the class Mollicutes associated to the respiratory-reproductive complex in horses from Mayabeque, Cuba

Article Sidebar

PDF (Español (España)) HTML (Español (España)) XML (Español (España)) EPUB (Español (España))
Published: Dec 1, 2019
Keywords:
Acholeplasma, diagnosis, equine, Mollicutes, Mycoplasma

Main Article Content

Ania Ramón-Martínez
Centro Nacional de Sanidad Agropecuaria
Fulgencio Pupo-Batista
Universidad Agraria de La Habana
Lisset Roblejo-Arias
Centro Nacional de Sanidad Agropecuaria
Adrian Díaz Sánchez
Centro Nacional de Sanidad Agropecuaria
Ileana Miranda-Cabrera
Centro Nacional de Sanidad Agropecuaria
Belkis Corona González
Centro Nacional de Sanidad Agropecuaria
Evelyn Lobo-Rivero
Centro Nacional de Sanidad Agropecuaria

Abstract

Researches on the detection of mycoplasmas in horses are relatively scarce. However, there are reports about their association to the respiratory-reproductive complex. In Cuba, the presence of these microorganisms in horses is unknown, so the objective of this work was to detect the presence of microorganisms of the class Mollicutes associated to the respiratory-reproductive complex in horses from Mayabeque, Cuba complex in horses from San José de las Lajas, Mayabeque. For this purpose, 97 animals from four localities of this municipality were analyzed. One hundred and thirty-one exudates (97 nasal and 34 preputial) were collected for diagnosis by microbiological culture and PCR. The presence of Mollicutes was detected in 37.11 % of the samples; of these 88.8 % corresponded to nasal exudates and 11.2 % to preputial exudates. The presence of Mollicutes was detected in all localities. Locality "D" and animals older than two years old had the highest number of positive results. An isolate was obtained from preputial exudate, which was identified as Acholeplasma spp. by biochemical tests. Studies related to the presence of Mollicutes in horses are being carried out for the first time in Cuba, so it is necessary to continue studies related to the epidemiology of these pathogens, as part of the control of the respiratory-reproductive complex in this species.

Article Details

How to Cite
1.
Ramón-Martínez A, Pupo-Batista F, Roblejo-Arias L, Díaz Sánchez A, Miranda-Cabrera I, Corona González B, Lobo-Rivero E. Detection of microorganisms of the class Mollicutes associated to the respiratory-reproductive complex in horses from Mayabeque, Cuba. Rev. Salud Anim. [Internet]. 2019 Dec. 1 [cited 2024 Nov. 22];41(3). Available from: https://revistas.censa.edu.cu/index.php/RSA/article/view/1039
Issue
Vol. 41 No. 3 (2019): septiembre-diciembre
Section
ARTÍCULOS ORIGINALES
National Center for Animal and Plant Health (CENSA)

References

Cordova C, Hoeltgebaum DL, Machado L, Dos Santos L. Molecular biology of mycoplasmas: from the minimum cell concept to the artificial cell. Annals of the Brazilian Academy of Sciences. 2016:599-607.

Nocard R. The microbe of pleuropneumonia. 1896. Rev Infect Dis. 1990;12(2):354-358.

Johansson KE, Pettersson B. Taxonomy of Mollicutes. In: Razin R, editor. Molecular Biology and Pathogenicity of Mycoplasmas. USA: Springer.2008. p. 29.

Champoux J, Neidhardt F, Drew LD, Plorde JJ. Sherris Medical Microbiology: An Introduction To Infectious Diseases. 4TH ed. Ray KJRyCG, editor. 2004. 409-13 p.

Bradbury J. Micoplsamas aviares: situación epidemiológica actual, bioseguridad y diagnóstico. 2007:6.

Fernández-Molina C, Rodríguez-Preval N, Rodríguez-González I, Agnese-Latino M, Rivera-Tapia JA, Ayala-Rodríguez I. Diagnóstico de Mycoplasma genitalium por amplificación de los genes MgPa y ARN ribosomal 16S. Salud Pública de México. 2008;50(5):358-61.

Razin S, Hayflick L. Highlights of mycoplasma research - An historical perspective. Biologicals. 2010;38(2):183-190.

Manual de la Organización Mundial de Sanidad Animal (OIE). 2015.

Informe semestral de la Dirección de Sanidad Animal, MINAG. 2017.

Spergser J, Aurich C, Aurich JE, Rosengarten R. High prevalence of mycoplasmas in the genital tract of asymptomatic stallions in Austria. Vet Microbiol. 2002;87(2):119-129.

Mete A, Özgür NY. Investigation of the presence of Mycoplasma as an etiologic agent of inflammatory airway diseases in thoroughbred racehorses in Istanbul Province. Turk J Vet Anim Sci. 2017;41:365-371.

Malik P, Chahota R, Bhardwaj B, Gupta S, Sharma M. A Study on the Prevalence of Mycoplasma spp. in the Upper Respiratory Tract of Healthy and Diseased Equines in Himachal Pradesh. 2016; p. 31.

Losinno L, Córdoba ML, Fumuso E. Infecciones de transmisión sexual (ITS) en equinos. 2016;1-32.

Nehra K, Rana R, Viswas KN, Arun TR, Singh VP, Singh AP, et al. Isolation and molecular identification of Mycoplasma equigenitalium from equine genital tracts in northern India. Iran J Vet Res. 2015;16(2):176-181.

Poveda JB. Biochemical Characteristic in Mycoplasma identification. In: Miles RJ, Nicholas RA, editors. Methods in Molecular Biology. Totowa, New Jersey, U.S.A: Humana Press. Inc.; 2010; p. 69-79.

Razin S, Tully J, Rose D, Barile M. DNA cleavage patterns as indicators of genotypic heterogeneity among strains of Acholeplasma and Mycoplasma species. J Gen Microbiol. 1983;129(6):1935-1944.

Freundt E. Culture media for classic mycoplasmas. Methods in mycoplasmology. 1983;1:127-35.

Fernández C, Chávez Y. Aplicación de la Reacción en Cadena de la Polimerasa para la detección de micoplasmas en cultivos celulares. Rev Salud Anim. 1999;18(1):31-34.

van Kuppeveld FJ, Johansson KE, Galama JM, Kissing J, Bolske G, van der Logt JT, et al. Detection of mycoplasma contamination in cell cultures by a mycoplasma group-specific PCR. Applied Environmental Microbiol. 1994;60(1):149-152.

European Pharmacopoeia 8.0, section 2.6.7. Mycoplasmas. 2014.

Molla KV, Shokrgozar MA, Arabestani MR, Moghadam MS, Azari S, Maleki S, et al. PCR-based detection and eradication of mycoplasmal infections from various mammalian cell lines: a local experience. Cytotechnology. 2009;61(3):117-124.

Castillo YIM. COMPAPROP: Sistema para comparación de proporciones múltiples. Rev Protección Veg. 2014;29(3):231-234.

Carman S, Rosendal S, Huber L, Gyles C, McKee S, Willoughby RA, et al. Infectious agents in acute respiratory disease in horses in Ontario. Journal of veterinary diagnostic investigation: official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 1997;9(1):17-23.

Antal T, Szabo I, Antal V, Vajda G, Polner A, Totth B, et al. Respiratory disease of horses associated with Mycoplasma infection. Zentralblatt fur Veterinarmedizin Reihe B J Vet Med, Series B. 1988;35(4):264-270.

Hasselbring BM, Sheppard ES, Krause DC. P65 Truncation Impacts P30 Dynamics during Mycoplasma pneumoniae Gliding. J Bacteriol. 2012;194(11):3000-3007.

Pilo P, Vilei EM, Peterhans E, Bonvin-Klotz L, Stoffel MH, Dobbelaere D, et al. A metabolic enzyme as a primary virulence factor of Mycoplasma mycoides subsp. mycoides small colony. J Bacteriol. 2005;187(19):6824-6831.

Dieckmann SM, Winkler M, Groebel K, Dieckmann MP, Hofmann-Lehmann R, Hoelzle K, et al. Haemotrophic Mycoplasma infection in horses. Vet Microbiol. 2010;145(3-4):351-353.

Baczynska A, Fedder J, Schougaard H, Christiansen G. Prevalence of mycoplasmas in the semen and vaginal swabs of Danish stallions and mares. Vet Microbiol. 2007;121(1-2):138-143.

Pereyra NB, Pérez AM, Messick JB, Cane FD, Guglielmone AA. Estudio de factores de riesgo asociados a la infección por Mycoplasma suis. InVet. 2010;12(2):121-130.

Waites KB, Katz B, Schelonka RL. Mycoplasmas and ureaplasmas as neonatal pathogens. Clin Microbiol Rev. 2005;18(4):757-789.

Volokhov DV, Graham LJ, Brorson KA, Chizhikov VE. Mycoplasma testing of cell substrates and biologics: Review of alternative non-microbiological techniques. Mol Cell Probes. 2011;25(2-3):69-77.

Brown DR. Phylum XVI. Tenericutes. In: De Vos P, G. Garrity, D. Jones, N.R. Krieg, W. Ludwig, F.A. Rainey, K.H. Schleifer y W.B. Whitman, editor. Bergey's Manual(r) of Systematic Bacteriology: Volume Four The Bacteroidetes, Spirochaetes, Tenericutes (Mollicutes), Acidobacteria, Fibrobacteres, Fusobacteria, Dictyoglomi, Gemmatimonadetes, Lentisphaerae, Verrucomicrobia, Chlamydiae, and Planctomycetes: Springer-Verlag New York. 2010; p. 976.

Lobo E. Mollicutes de interés en Medicina Veterinaria. Monografías. 2004.

Kirchhoff H, Naglic T, Heitmann J. Isolation of Acholeplasma laidlawii and Mycoplasma equigenitalium from stallion semen. Vet Microbiol. 1979;4:177-179.

Most read articles by the same author(s)

> >>