Molecular detection of Leptospira spp. and Mycobacteriums spp. in cattle of Los Palmitos municipality, Sucre - Colombia

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Published: Apr 1, 2020
Keywords:
cattle, Leptospira spp., Mycobacterium spp., Dietzia, 16S rRNA, PCR

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Lina Lineth Durán Cueto
Universidad de Sucre
Libardo Enrique Caraballo Blanco
Universidad de Sucre
Pedro José Blanco Tuirán
Universidad de Sucre

Abstract

The objective of the present study was to detect the presence of Leptospira spp. and Mycobacterium spp. by means of molecular techniques in cattle of Los Palmitos municipality, Sucre-Colombia.. One hundred seventy-two blood samples from individuals from 12 farms were studied. They were analyzed by polymerase chain reaction (PCR) with two pairs of primers amplifying a region of the 16S rRNA gene of Mycobacterium spp. and other primers specific for Leptospira DNA gyrase subunit B (gyrB). The segment of interest of the 16S rRNA gene was amplified from 35 samples (20 %), of these, 12 were sent for sequencing. Only eight sequences were informative, of which six belonged to species of the genus Dietzia and two to the genus Mycobacterium. Of the three positive samples to Leptospira spp. (1.7 %), only one was recovered showing identity with the species Leptospira interrogans. These results allow to affirm that cattle in Los Palmitos, Sucre-Colombia, are carriers of bacterial species that can generate a negative impact on livestock production, being also a risk to human health.

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How to Cite
1.
Durán Cueto LL, Caraballo Blanco LE, Blanco Tuirán PJ. Molecular detection of Leptospira spp. and Mycobacteriums spp. in cattle of Los Palmitos municipality, Sucre - Colombia. Rev. Salud Anim. [Internet]. 2020 Apr. 1 [cited 2024 Nov. 21];42(1). Available from: https://revistas.censa.edu.cu/index.php/RSA/article/view/1065
Issue
Vol. 42 No. 1 (2020): enero-abril
Section
ARTÍCULOS ORIGINALES
National Center for Animal and Plant Health (CENSA)

References

Michel G, Reyes J, Michel H, Davila R, Rocha G, Iñiguez A, et al. Módulo de clínica de bovinos: Enfermedades bacterianas endémicas primera edición ed. Jalisco, México.2013.

Guerra M. Leptospirosis: public health perspectives. Biologicals 41: 295-297. 2013(1095-8320 ).

Betancur C, Orrego A, González M. Seroepidemiología de la leptospirosis en bovinos con trastornos reproductivos en el municipio de Montería. Rev Med Vet. 2013;(26):47-55.

Adler B, de la Pena Moctezuma A. Leptospira and leptospirosis. Veterinary microbiology. 2010.

Fornazari F, da Silva R, Richini V, Beserra H, Luvizotto M, Langoni H. Comparison of conventional PCR, quantitative PCR, bacteriological culture and the Warthin Starry technique to detect Leptospira spp. in kidney and liver samples from naturally infected sheep from Brazil. J Microbiol Methods. 2012;90(3):321-326.

Langoni H, Souza L, Silva A, Luvizotto M, Paes A, Lucheis S. Incidence of leptospiral abortion in Brazilian dairy cattle. Prev Vet Med. 1999;40:271-275.

Kazda J, Pavlik I, Falkinham J, Hruska K. The Ecology of Mycobacteria: Impact on Animal's and Human's Health. 2009.

Romero B. Tuberculosis bovina: epidemiología molecular y su implicación en Sanidad animal y Salud Píblica Madrid: Universidad Complutense de Madrid.2012.

Jelalu K, Berhanu S, Aklilu A, Yitagele T, Ketema T, Kiros W, et al. Bovine tuberculosis in eastern Ethiopia: prevalence, risk factors and its public health importance. BMC Infectious Diseases. 2019;19(1):39.

Sweetline A, Ronald B, Senthil K, Thangavelu A. Conventional and molecular determination of drug resistance in Mycobacterium tuberculosis and Mycobacterium bovis isolates in cattle.Tuberculosis (Edinburgh, Scotland).2019;114:113-118.

Iturra L. Evaluación del impacto económico de la erradicación de tuberculosis bovina en predios lecheros infectados. Chile: Universidad de Chile. 2016.

Douarre PE, Cashman W, Buckley J, Coffey A, O'Mahony JM. Isolation and detection of Mycobacterium avium subsp. paratuberculosis (MAP) from cattle in Ireland using both traditional culture and molecular based methods. Gut Pathogens.2010;2(1):11.

Dziedzinska R, Slana I. Mycobacterium avium subsp. paratuberculosis - An Overview of the Publications from 2011 to 2016. Current Clinical Microbiol Reports. 2017;4(1):19-28.

Aguilera A. Economía Regional: La Economía del Departamento de Sucre: Ganadería y Sector Público. Colombia. 2005.

Codazzi IgA. Diccionario Geográfico de Colombia Tercera edición ed. Colombia. 1996.

Miller SA, Dykes DD, Polesky HF. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Research. 1988;16(3):1215.

Tevere V, Hewitt P, Dare A, Hocknell P, Keen A, Spadoro J, et al. Detection of Mycobacterium tuberculosis by PCR Amplification with Pan-Mycobacterium Primers and Hybridization to an M. tuberculosis-Specific Probe. J Clinical Microbiol. 1996;34(4):918-923.

Slack A, Symonds M, Dohnt M, Smythe L. Identification of pathogenic Leptospira species by conventional or real-time PCR and sequencing of the DNA gyrase subunit B encoding gene. J BMC Microbiol. 2006;6:95.

Sanger F, Nicklen S, Coulson A. DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences of the United States of America. 1977;74(12):5463-5467.

Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. 2016.

Altschul S, Gish W, Miller W, Myers E, Lipman D. Basic local alignment search tool. Molecular Biology. 1990;215(3):403-410.

Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution. 1980;16(2):111-120.

Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular biology and evolution. 2013;30(12):2725-2729.

Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution. 1987;4(4):406-425.

García P, García L. Infecciones por micobacterias de crecimiento rápido. Enferm Infecc Microbiol Clin. 2012;30(4):162-200.

Lourenço C, Whipps C, Kayoko C, Chimara E, Droz S, Tortoli E, et al. Mycobacterium saopaulense sp. nov., a rapidly growing mycobacterium closely related to members of the Mycobacterium chelonae-Mycobacterium abscessus group. Syst Evol Microbiol. 2015;65(12):4403:4409.

Camarena J, González R. Micobacterias atípicas y su implicación en patología infecciosa pulmonar. Enfermedades infecciosas y microbiologia clinica. 2011;29(5):66-75.

Wang L-T, Lee F-L, Tai C-J, Kasai H. Comparison of gyrB gene sequences, 16S rRNA gene sequences and DNA-DNA hybridization in the Bacillus subtilis group. Systematic and Evolutionary Microbiology. 2007;57:1846-1850.

Fernández M. Identificación y poder patógeno de microrganismos del género "corynebacterium" aislados de muestras clínicas. Madrid: Complutense de Madrid; 2009

Koerner R, Goodfellow M, Jones A. The genus Dietzia : a newhomefor someknownand emerging opportunist pathogens. FEMS Immunol Med Microbiol. 2009;55:296-305.

Hamid ME. Dietzia species as a cause of mastitis: Isolation and identification of five cases from dairy cattle. academic. 2013;7(29):3853-3857.

Caraballo L, Castellar A, Pardo E. Mycobacterium avium subespecie paratuberculosis en heces de bovinos del municipio de Sincelejo, Sucre, Colombia. Inv Vet Perú. 2018;29(3):987-995.

Mendoza J, Lana R, Díaz M. Mycobacterium avium subspecies paratuberculosis and its relationship with Crohn's disease. World J Gastroenterol. 2009;1r5(4).

Sweetline A, Ronald B, Kumar T, Kannan P, Thangavelu A. Molecular identification of Mycobacterium tuberculosis in cattle. Vet Microbiol. 2017;198:81-87.

Uzoigwe J, Khaitsa M, Gibbs P. Epidemiological evidence for Mycobacterium avium subspecies paratuberculosis as a cause of Crohn's disease. Epidemiol Infect. 2007;135(7):1057-1068.

Johnson M, Smith H, Joseph P, Gilman, Bautista C, Campos K, et al. Environmental Exposure and Leptospirosis. Peru Emerg Infect Dis. 2004;10(6):1016-1022.

Jafari Dehkordi A, Shahbazkia H, N. R. Evaluation of pathogenic serovars of Leptospira interrogans in dairy cattle herds of Shahrekord by PCR. Microbiol. 2011;3(3):135-139.

Britton L, Cassidy J, O'Donovan J, Gordon S, Markey B. Potential application of emerging diagnostic techniques to the diagnosis of bovine Johne's disease (paratuberculosis). Vet J. 2016;209:32-39.