Management of Aethina tumida (Murray) with Beauveria bassiana (Bals) and Metarhizium anisopliae (L.) In vivo assay
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Abstract
The work was carried out at Laboratory III, Agricultural Sciences Faculty, University of Sancti Spíritus. Its aim was to determine the effect of four doses of strains LBb-111 and LBb-1234 of Beauveria bassiana Bals. and Ma-34 of Metarhyzum anisopliae L. on the mortality of Aethina tumida larvae and adults collected in infected beehives. A mixture of 375 g of soil + 100 g of washed sand + four doses of entomopathogens was used for the experiment. Then, 500 g of the mixture were placed in boxes (6.2 cm high x 15.5 cm diameter). Ten larvae/boxes/dose were placed in a first assay and 10 adults/boxes/dose in others, with seven replicates per treatment, respectively. Mortality percentages were determined every 24 hours and a factorial analysis was carried out with an interaction between the doses and the strains used. As a result, Strain LBb-111 of B. bassiana and Ma-34 of M. anisopliae reached the highest percentage of adult mortality with 40g and 60g, with values higher than 50 % in 96 hours. The highest percentage of larval mortality was reached with the 60g dose with strain LBb-111 of B. bassiana in 96 hours.
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National Center for Animal and Plant Health (CENSA)References
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