Main morphological alterations in ovine oocytes matured in vitro

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Published: Dec 1, 2018
Keywords:
oocyte alterations, morphological evaluation, in vitro maturation, ovine

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Sarahí Hernández-Martínez
Universidad Autónoma Metropolitana
María del Carmen Navarro-Maldonado
Universidad Autónoma Metropolitana
Demetrio Alonso Ambríz-García
Universidad Autónoma Metropolitana
José Roberto Vázquez-Avendaño
Universidad Autónoma Metropolitana
José Ernesto Hernández-Pichardo
Universidad Autónoma Metropolitana

Abstract

The oocyte is indispensable for the embryonic development of an organism. Its quality is currently evaluated considering the morphological parameters that allow identifying the alterations that compromise the development of the embryo. So far, no morphological abnormalities have been reported in sheep oocytes. The objective of this study was to determine the frequency and type of morphological alterations in them. For this, 10 collections were carried out, of which 567 ovaries were obtained from slaughtered females and 2 374 oocytes were obtained by follicular aspiration; all of them matured in vitro. According to the criteria of the Association for the Study of Reproductive Biology (ASEBIR), 798 oocytes (33.6 %) were classified as normal morphology and 1 576 (66.4 %) presented different morphological alterations, as granular cytoplasm (32.6 %), incomplete cytoplasm (21.1 %), increased perivitelline space (11.7 %), presence of vacuoles (11.6 %), degenerated oocytes (8.9 %), abnormalities of the pellucid zone ( 7 %), cytoplasm exudates (4.7 %), polar body dimorphism (0.3 %), and alterations produced by the management technique, like the absence of pellucid zone (2.1 %). It is concluded that, given the high rate of morphological abnormalities in the in vitro maturation of oocytes, their detailed selection is important to obtain better results in the in vitro embryonic development.

Article Details

How to Cite
1.
Hernández-Martínez S, Navarro-Maldonado M del C, Ambríz-García DA, Vázquez-Avendaño JR, Hernández-Pichardo JE. Main morphological alterations in ovine oocytes matured in vitro. Rev. Salud Anim. [Internet]. 2018 Dec. 1 [cited 2024 Nov. 22];40(3). Available from: https://revistas.censa.edu.cu/index.php/RSA/article/view/994
Issue
Vol. 40 No. 3 (2018): septiembre-diciembre
Section
ARTÍCULOS ORIGINALES
National Center for Animal and Plant Health (CENSA)

References

Aguilar-Piña RE. El regreso del ovocito: de la olvidada transferencia citoplasmática a la actual transferencia del huso meiótico. Rev Mex Med Reprod. 2012;4(3):132-138.

ASEBIR. Asociación para el Estudio de la Biología de la Reproducción, Criterios ASEBIR de valoración morfológica de ovocitos, embriones tempranos y blastocistos humanos. Cuadernos de embriología clínica, 3rª edición, España. 2015.

Ingilizova G, Ivanov D, Kovachev E, Evrev M, Kostov I, Necheva V. Oocyte quality as a predictive marker for assessment of IVF/ICSI procedure outcome. Akush Ginekol. 2014;53(6):41-46.

Palma G. Biotecnología de la Reproducción, Ediciones: Instituto Nacional de Tecnología Agropecuaria, 1ª edición, Argentina. 2001.

Robledo-Verduzco JM, Herrera-Camacho J, Cajero-Juárez M, Navarro-Maldonado MC, García-Valladares A. Evaluación de dos medios de maduración in vitro para la producción de embriones ovinos. Trop Subtrop Agroecosyst. 2004;10(1):95-99.

Kakkassery MP, Vijayakumaran V, Sreekumaran T. Effect of cumulus oocyte complex morphology on in vitro maturation of bovine oocytes. J Vet Anim Sci. 2010;41:12-17.

Rodríguez-Suástegui JL, Romo-García S, Ducolomb I, Casas-Hernández E, Hernández-Pichardo J. Desarrollo de mórulas de ovino en medio simple o secuencial: relación entre evaluación morfológica y viabilidad embrionaria. Rev Salud Anim. 2017;39(1):9-18.

Kragh PM, Vajta G, Corydon TJ, Purup S, Bolund L, Callesen H. Production of transgenic porcine blastocysts by hand-made cloning. Reprod Fertil Dev. 2004;16(3):315-318.

Dal Canto M, Guglielmo MM, Mignini RM, Fadini R, Moutier C, Merola M, et al. Dysmorphic patterns are associated with cytoskeletal alterations in human oocytes. Hum Reprod. 2017;32(4):750-775

Faramarzi A, Mohammad AK, Ashourzadeh S. Oocyte morphology and embryo morphokinetics in an intra-cytoplasmic sperm injection programme. Is there a relationship? Zygote. 2017;7:1-7.

Palmerini MG, Nottola SA, Leoni GG, Succu S, Borshi X, Berlinguer F, et al. In vitro maturation is slowed in prepubertal lamb oocytes: ultrastructural evidences. Reprod Biol Endocrinol. 2014;12:115.

Men H, Monson RL, Parrish JJ, Rutledge JJ. Degeneration of cryopreserved bovine oocytes via apoptosis during subsequent culture. Cryobiology. 2003;47(1):73-81.

Wallbutton S, Kasraie J. Vacuolated oocytes: fertilization and embryonic arrest following intra-cytoplasmic sperm injection in a patient exhibiting persistent oocyte macro vacuolization-Case report. J. Assist Reprod Genet. 2010;27(4):183-188.

Sousa M, Cunha M, Silva J, Oliveira E, Joao M, Almeida C, et al. Ultrastructural and cytogenetic analyses of mature human oocyte dysmorphisms with respect to clinical outcomes. J Assist Reprod Genet. 2016;33:1041-1057.

Yu EJ, Ahn H, Lee JM, Jee BC, Kim SH. Fertilization and embryo quality of mature oocytes with specific morphological abnormalities. Clin Exp Reprod Med. 2015;42:156-162.

Reader KL, Cox NR, Stanton J-AL, Juengel JL. Effects of acetyl L-carnitine on lamb oocyte blastocyst rate, ultrastructure and mitochondrial DNA copy number. Theriogenology. 2015;83(9):1484-1492.

Otsuki J, Iwasaki T, Katada Y, Tsutsumi Y, Tsuji Y, Furuhashi K, et al. A higher incidence of cleavage failure in oocytes containing smooth endoplasmic reticulum clusters. J Assist Reprod Genet. 2018;35(5):899-905.

Stigliani S, Moretti S, Casciano I, Canepa P, Remorgida V, Anserini P, et al. Presence of aggregates of smooth endoplasmic reticulum in MII oocytes affects oocyte competence: molecular based evidence. Mol Hum Reprod. 2018;24(6):310-317.

Ebner T, Moser M, Sommergruber M, Yaman C, Pfleger U, Tews G. First polar body morphology and blastocyst formation rate in ICSI patients. Hum Reprod. 2002;17:2415-2418.

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