In vitro-fertilized bovine embryos co-cultured with oviductal and cumulus oophorus cells

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Published: Mar 15, 2019
Keywords:
embryo development, bovine, in vitro, co-culture

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Yury Grisel Soto-Martínez
Universidad Autónoma Metropolitana-Iztapalapa
Eduardo Casas-Hernández
Universidad Autónoma Metropolitana-Iztapalapa
José Miguel Betancourt-Rule
Universidad Autónoma Metropolitana-Iztapalapa
Filiberto Fernández-Reyes
Universidad Autónoma Metropolitana-Xochimilco

Abstract

The research was aimed at evaluating the effect of embryos co-cultured with oviductal and cumulus oophorus cells on the development of blastocyst in vitro. Oviducts and ovaries were collected from slaughtered bovine females. Oviductal cells were obtained after a longitudinal incision of the oviduct and cumulus cells were collected from follicular fluid; both cell types were placed for culture in TCM-199 with 20 % Fetal Bovine Serum (FBS). The cumulus-oocyte complexes (COC's) were collected by the aspiration of follicles from 2 to 7 mm diameter and they were placed for maturation in TCM-199 with 10 % FBS. Subsequently, they were fertilized using a concentration of 1x106 spermatozoa/mL. After fertilization, they were divided into three treatments: 1) Control, 2) Co-culture with cumulus cells and 3) Co-culture with oviduct cells. The development was evaluated at 192 hours of in vitro culture, determining the amount of divided embryos, morulas and blastocysts. They were cultured in sequential SOF medium by the treatment: 1) 538 oocytes, 2) 627 oocytes and 3) 478 oocytes. The results were the following: 14 % of blastocysts in the co-culture of cumulus cells, 7 % in the co-culture of oviduct and 4 % in the control, with a significant statistical difference (p<0.05) among the three treatments. It is concluded that the use of somatic cells in conditioned medium can stimulate the blastocyst stage development in vitro to the bovine embryos.

Article Details

How to Cite
1.
Soto-Martínez YG, Casas-Hernández E, Betancourt-Rule JM, Fernández-Reyes F. In vitro-fertilized bovine embryos co-cultured with oviductal and cumulus oophorus cells. Rev. Salud Anim. [Internet]. 2019 Mar. 15 [cited 2024 Nov. 21];41(1). Available from: https://revistas.censa.edu.cu/index.php/RSA/article/view/999
Issue
Vol. 41 No. 1 (2019): January-April
Section
ARTÍCULOS ORIGINALES
National Center for Animal and Plant Health (CENSA)

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