In vitro-fertilized bovine embryos co-cultured with oviductal and cumulus oophorus cells

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Published: Mar 15, 2019
Keywords:
embryo development, bovine, in vitro, co-culture

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Yury Grisel Soto-Martínez
Universidad Autónoma Metropolitana-Iztapalapa
Eduardo Casas-Hernández
Universidad Autónoma Metropolitana-Iztapalapa
José Miguel Betancourt-Rule
Universidad Autónoma Metropolitana-Iztapalapa
Filiberto Fernández-Reyes
Universidad Autónoma Metropolitana-Xochimilco

Abstract

The research was aimed at evaluating the effect of embryos co-cultured with oviductal and cumulus oophorus cells on the development of blastocyst in vitro. Oviducts and ovaries were collected from slaughtered bovine females. Oviductal cells were obtained after a longitudinal incision of the oviduct and cumulus cells were collected from follicular fluid; both cell types were placed for culture in TCM-199 with 20 % Fetal Bovine Serum (FBS). The cumulus-oocyte complexes (COC's) were collected by the aspiration of follicles from 2 to 7 mm diameter and they were placed for maturation in TCM-199 with 10 % FBS. Subsequently, they were fertilized using a concentration of 1x106 spermatozoa/mL. After fertilization, they were divided into three treatments: 1) Control, 2) Co-culture with cumulus cells and 3) Co-culture with oviduct cells. The development was evaluated at 192 hours of in vitro culture, determining the amount of divided embryos, morulas and blastocysts. They were cultured in sequential SOF medium by the treatment: 1) 538 oocytes, 2) 627 oocytes and 3) 478 oocytes. The results were the following: 14 % of blastocysts in the co-culture of cumulus cells, 7 % in the co-culture of oviduct and 4 % in the control, with a significant statistical difference (p<0.05) among the three treatments. It is concluded that the use of somatic cells in conditioned medium can stimulate the blastocyst stage development in vitro to the bovine embryos.

Article Details

How to Cite
1.
Soto-Martínez YG, Casas-Hernández E, Betancourt-Rule JM, Fernández-Reyes F. In vitro-fertilized bovine embryos co-cultured with oviductal and cumulus oophorus cells. Rev. Salud Anim. [Internet]. 2019 Mar. 15 [cited 2024 Nov. 14];41(1). Available from: https://revistas.censa.edu.cu/index.php/RSA/article/view/999
Issue
Vol. 41 No. 1 (2019): January-April
Section
ARTÍCULOS ORIGINALES
National Center for Animal and Plant Health (CENSA)

References

Mucci N, Aller J, Kaiser G, Hozbor F, Alberio R. Producción in vitro de embriones bovinos: Suplementación de los medios de cultivo con suero. Arch Med Vet. 2006;38:97-104.

López A, Olivera M, Ruiz T, Tarazona A. Efecto del co-cultivo sobre el desarrollo temprano de embriones bovinos producidos in vitro. Rev. MVZ Córdoba. 2007; 12:1061-1067.

Ahuja C, Montiel F, Pérez P, Gallegos J. Medio alternativo para la producción in vitro de embriones bovinos. Zootec Trop. 2009; 27:277-284.

Hirayama H, Moriyasu S, Kageyama S, Sawai K, Takahashi H, Geshi M, et al. Enhancement of maternal recognition of pregnancy with parthenogenetic embryos in bovine embryo transfer. Theriogenology. 2014;81:1108-1115

Bang JI, Jin JI, Ghanem N, Choi BH, Fakruzzaman M, Ha AN, et al. Quality improvement of transgenic cloned bovine embryos using an aggregation method: Effects on cell number, cell ratio, embryo perimeter, mitochondrial distribution, and gene expression profile. Theriogenology. 2015;84(4):509-523.

Suttiroipattana T, Somfai T, Matoba S, Nagai T, Parnpai R, Geshi M. The effect of temperature during liquid storage of in vitro-matured bovine oocytes on subsequent embryo development. Theriogenology. 2016;85(3):509-518.

García EV, Hamdi M, Barrera AD, Sánchez-Calabuig MJ, Gutiérrez-Adán A, Rizos D. Bovine embryo-oviduct interaction in vitro reveals an early cross talk mediated by BMP signaling. Reproduction. 2017;153(5):631-643.

Valleh MV, Hyttel P, Rasmussen MA, Strøbech L. Insulin-like growth factor 2: A modulator of anti-apoptosis related genes (HSP70, BCL2-L1) in bovine reimplantation embryos. Theriogenology. 2014;82(7):942-950.

Tarazona A, Olivera-Angel M, Lenis Y. Rol de la mitocondria y el estrés oxidativo del desarrollo de embriones bovinos producidos in vitro. Arch Med Vet. 2010;42:125-133.

Fields S, Hansen P, Ealy A. Fibroblast growth factor requirements for in vitro development of bovine embryos. Theriogenology. 2011;75(8):1466-1475.

Kussano NR, Leme LO, Guimaräes AL, Franco MM, Dobe MA. Molecular markers for oocyte competence in bovine cumulus cells. Theriogenology. 2016;85(6):1167-1176.

Córdova A, Perreau C, Uzbekova S, Locatelli Y, Mermillo P. Development rate and gene expression of IVP bovine embryos cocultured with bovine oviduct epithelial cells at early or late stage of preimplantation development. Theriogenology. 2014;81:1163-1173

Aboelenain M, Balboula AZ, Kawahara M, El-Monem Monaster A, Zaabel SM, Kim SW, et al. Pyridoxine supplementation during oocyte maturation improves the development and quality of bovine preimplantation embryos. Theriogenology. 2017;91:127-133.

Lopera-Vasquez R, Hamdi M, Maillo V, Gutierrez-Adan A, Bermejo-Alvarez P, Ramírez MÁ, et al. Effect of bovine oviductal extracellular vesicles on embryodevelopmental and quality in vitro. Reproduction. 2017;153(4):461-470.

Da Costa NN, Brito KN, Santana PD, Cordeiro MD, Silva TV, Santos AX, et al. Effect of cortisol on bovine oocyte maturation and embryo development in vitro. Theriogenology. 2016;85(2):323-329.

Nuttinck F, Jouneau A, Charpigny G, Hue I, Richard C, Adenot P, et al. Prosurvival effect of cumulus prostaglandin G/H synthasa 2/prostaglandin2 signalig on bovine blastocyst: impact on in vivo posthatching development. Biol Reprod. 2017;96(3):531-541.

Moreno D, Neira A, Dubreil L, Destrumelle S, Michaud S, Thorin C, et al. In vitro bovine embryo production in a synthetic medium: Embryo development, cryosurvival, and establishment of pregnancy. Theriogenology. 2015;84(7):1050-1060.

Wale PL, Gardner DK. The effects of chemical and physical factor son mammalian embryo culture and their importance for the practice of assisted human reproduction. Hum Reprod Update. 2016;22(1):2-22.

Karami S, Shahsavari MH, Hajarian H, Moghaddam G. In vitro developmental competence of bovine oocytes: Effect of corpus luteum and follicle size. Iran J Reprod Med. 2015;23(10):615-622.

Goto K, Iwai N, Takuma Y, Nakanishi Y. Co-culture of in vitro fertilized bovine embryos with different cell monolayers. J Anim Sci. 1992;70:1449-1453.

Robertson I, Nelson R. Certification and identification of the embryo. In: Stringfellow DA, Seidel SM (Ed). Manual of International Embryo Transfer. Champaign, IL: IETS. 2011; pp.:103-117.

Sigma. Productos de investigación en ciencias de la vida. México. 2001;pp.:23-1013.

Hintze J. NCSS, PASS, and GESS. NCSS. Kaysville, Utah. http://www.ncss.com. 2006.

Ulbrich S, Zitta K, Hiendleder S, Wolf E. In vitro systems for intercepting early embryo-maternal cross-talk in the bovine oviduct. Theriogenology. 2010;73:802-816.

Mejía V, Arango S, Pareja A, Camargo O, Urrego R. Comparison of two culture media over the IVP of bovine embryos. Revista CES. Medicina Veterinaria y Zootecnia. 2009;4:39-46.

Bilodeau S, Panich P. Effects of oocyte quality on development and transcriptional activity in early bovine embryos. Anim Reprod Sci. 2002;71:143-155.

Zuccotti M, Merico V, Cecconi S, Redi C, Garagna S. What does it take to make a developmentally competent mammalian egg? Human Reprod Update. 2011;17(4):525-540.

Sun WJ, Pang YW, Liu Y, Hao HS, Zhao XM, Qin T, et al. Exogenous glutathione supplementation in cultura medium improves the bovine embryo development after in vitro fertilization. Theriogenology. 2015;84(5):716-723.

Chen H, Zhang L, Guo Z, Wang Y, He R, Qin Y, et al. Improving the development of early bovine somatic-cell nuclear transfer embryos by treating adult donor cells with vitamin C. Mol Reprod Dev. 2015;82(11):867-879.

Urania F, Dovolou E, Rekkas CA, Heras S, Pappas I, Soom AV, et al. Urokinasa-type plasminogen activator dose not affect in vitro bovine embryo development and quality. Act Vet Hung. 2015;63(2):243-254.

Khan DR, Guillemette C, Sirard MA, Richard FJ. Transcriptomic analysis of ciyclic AMP response in bovine cumulus cells. Physiol Genomics. 2015;47(9):432-442.

Pinyopummintry T, Bavister B. In vitro-maturated/in vitro-fertilized bovine oocytes can develop into morulae/blastocyst in chemically defined, protein-free culture media. Biol Reprod. 1991;45:736-742.

Jordanes L, Arias-Alvarez M, Pintelon I, Thys S, Valckx S, Dezhkam Y, et al. Elevated non-esterified fatty acid concentrations hamper bovine oviductal epithelial cell physiology in three different in vitro culture sistems. Theriogenology. 2015;84(6):899-910.

Kropp J, Khatib H. Characterization of microRNA in bovine in vitro culture media associated with embryo quality and development. J dairy Sci. 2015;98(9):6552-6563.

Wdowiak A, Bakalczuk S, Bakalczuk G. The effect of sperm DNA fragmentation on the dynamics of the embryonic development in intracytoplasmatic sperm injection. Reprod Biol. 2015;15(2):94-100.

Shirazi A, Ostad S, Ahmadi E, Heidari B, Shams N. In vitro development competence of ICSI-derived activated ovine embryos. Theriogenology. 2009;71:342-348.

Orsi M, Reischl J. Mammalian embryo co-culture: Trials and tribulations of a misunderstood method. Theriogenology. 2007;67:441-458.

Schmaltz-Panneau B, Locatelli Y, Uzbekova S, Perreau C, Mermillod P. Bovine oviduct epithelial cells differentiate partly in culture, while maintaining their ability to improve early embryo development rate and quality. Reprod Domest Anim. 2015;50(5):719-729.

Familiari G, Heyn R, Relucenti M, Nottola S, Sathananthan A. Ultrastructural Dynamics of Human Reproduction, from Ovulation to Fertilization and Early Embryo Development. International Review of Cytology. 2006;249:53-141.

Shirazi A, Moalemian Z. Ovine cumulus cells estradiol-17ß production in the presence or absence of oocyte. Anim Reprod Sci. 2007;101:125-133.

Ferguson C, Kesler D, Godke R. Progesterone enhances in vitro development of bovine embryos. Theriogenology. 2012;77:108-114.

Rief S, Sinowatz F, Stojkovic M, Einspanier R, Wolf E, Prelle K. Effects of a novel co-culture system on development, metabolism and gene expression of bovine embryos produced in vitro. Reprod. 2002;124:543-556.

Gardner D, Lane M. Principles of Cloning. Chapter 6. Culture of viable mammalian embryos in vitro. Second Edition. Elsevier. USA. 2014;pp.63-84.

Schoen J, Bondzio A, Topp K, Einspanier R. Establishment and characterization of an adherent pure epithelial cell line derived from the bovine oviduct. Theriogenology. 2008;69:536-545.

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