Obtención por vía recombinante de dos citadhesinas de Mycoplasma gallisepticum

José Antonio Agüero-Fernández, Andy Potter, José Pérez-Casal

Resumen

Los micoplasmas son los organismos autorreplicativos más simples que se conocen y se distinguen de otras bacterias por su pequeña talla y la total carencia de pared celular. La industria avícola está afectada por varias especies de micoplasmas, pero Mycoplasma gallisepticum (MG) es el más significativo económicamente. La adherencia de los micoplasmas a las células epiteliales respiratorias del hospedero constituye un paso crítico para la infección y posterior desarrollo de la enfermedad y se realiza mediante la interacción de lipoproteínas localizadas en la superficie de la bacteria con los receptores de las células del hospedero. Las proteínas GapA y CrmA son indispensables en este proceso de adherencia y en el desarrollo posterior de la enfermedad. En este trabajo se describe el clonaje molecular de los genes que codifican para ambas proteínas, así como su expresión, purificación y la antigenicidad de los productos obtenidos. Se clonó solo un fragmento del gen gapA, mientras que crmA se clonó en toda su extensión. En ambos casos las proteínas recombinantes se obtuvieron fragmentadas, lo que no afectó el proceso de purificación por Cromatografía de Afinidad por Metales Inmovilizados (IMAC). Al final del proceso los productos recombinantes conservaron sus propiedades antigénicas. Estos productos recombinantes podrían ser empleados en futuros estudios relacionados con los mecanismos de patogenicidad de esta bacteria.

Palabras clave

Mycoplasma gallisepticum; proteína de superficie; proteína recombinante; purificación por IMAC

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Referencias

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