Susceptibility of Cosmopolites sordidus (Germar) adults to Heterorhabditis amazonensis Andaló et al. strain HC1

Main Article Content

Dairys García-Perera
Roberto Enrique
Lidia López
Daine Hernández-Ochandía
Ileana Miranda
Giselle Calabuche-Gómez
Oriela Pino
Lester Pupiro
Mayra G. Rodríguez

Abstract

The objective of this study was to determine the in vitro susceptibility of weevil adults (Cosmopolites sordicus Germar) to infective juveniles (IJ) of Heterorhabditis amazonenesis Andaló et al. (Cuban strain HC1). The adults were collected in the field with sandwich and modified disc traps and kept in quarantine in the laboratory until their use in the tests. The insects were inoculated with suspensions of infective juveniles at the dosage of 125, 250, 500, 2500, and 5000 JI.ml-1. Controls without inoculation were included. Mortality was evaluated from 12 hours to 21 days after inoculation. Lethal dose values (LD50, DL90) and lethal times (LT50 and LT90) were determined through Probit Analysis and the percentage of mortality by performing an Variance Analysis. The weevil adults killed by the effect of the nematodes became stiff and hard, maintained coloration and shrank their legs. The LD 50 and LD 90 determined were 1872 and 6061 IJ / ml-1, respectively. The LT values estimated using the concentration of 5000 IJ / ml-1 for the control of 50 and 90 % of a population of C. sordidus were 17 and 28 days, respectively. The highest percentage of mortality was 86.7 using the highest dose at 21 days.

Article Details

How to Cite
García-Perera, D., Enrique, R., López, L., Hernández-Ochandía, D., Miranda, I., Calabuche-Gómez, G., Pino, O., Pupiro, L., & Rodríguez, M. G. (2020). Susceptibility of Cosmopolites sordidus (Germar) adults to Heterorhabditis amazonensis Andaló et al. strain HC1. Revista De Protección Vegetal, 34(3). Retrieved from https://revistas.censa.edu.cu/index.php/RPV/article/view/1056
Section
SHORT COMMUNICATIONS

References

Gold CS, B Pinese, JE Peña. Pests of Banana. CAB International. Tropical Fruit Pests and Pollinators (eds J.E. Peña, J.L. Sharp, M. Wysoki). 2002. Pp 13- 56

Bortoluzzi L, Alves LFA, Alves VS, Holz N. Entomopathogenic nematodes and their interaction with chemical insecticide aiming at the control of banana weevil borer, Cosmopolites sordidus Germar (Coleoptera: Curculionidae). Arquivos do Instituto Biológico. 2013; 80(2):183-192.

Martínez González E, Barrios Sanromá G, Rovesti L, Santos Palma R. Bananos y plátanos (Musa paradisiaca y Musa sapientum Linnaeus). Manejo Integrado de Plagas. Manual Prático. Centro Nacional de Sanidad Vegetal (CNSV), Cuba. Entre pueblos, España. Grupo di Volontariato Civile (GVC), Italia (Eds.). 2006, pp.29-30.

Reinecke D. Distribución del picudo negro del plátano (Cosmopolites sordidus) en Cuba. Revista Especial Diez Anos de Colaboración Científica CUBA-RDA. Instituto de Investigaciones Fundamentales en Agricultura Tropical "Alejandro de Humboldt". Cuba. 1976: 52-56

Calderon A, Castineiras A, Lopez M. Efecto de los biocidas y fertilizantes empleados en el cultivo del plátano en Cuba sobre los hongos entomopatógenos. Rev. Agricultura. Serie Protección de Plantas (Cuba). 1991; 1: 21-31

Masso E, Neyra M. Daños y pérdidas causadas por Cosmopolites sordidus en el cultivo del plátano. Agrotecnia de Cuba. 1997; 27, 86-88.

Treverrow NL, Bedding RA. Development of a system for the control of the banana weevil borer, Cosmopolites sordidus, with entomopathogenic nematodes. En Nematodes and the biological Control of Insect Pests. Bedding R, Akhurst R, Kaya H K (Eds.), CSIRO, Melbourne, Australia.1993. pp. 41-47

Gold C S, J E Pena, E B Karamura. Biology and integrated pest management for the banana weevil Cosmopolites sordidus (Germar) (Coleoptera: Curculionidae). Integrated Pest Management Reviews. 2001; 6: 79-155

Stock SP. Diversity, Biology and Evolutionary Relationships. Pp 3- 28 En Nematode Pathogenesis of Insects and Other Pests. Sustainability in Plant and Crop Protection 1. R. Campos-Herrera (Ed.). Springer International Publishing Switzerland 2015. DOI 10.1007/978-3-319-18266-7_1

Kaya HK, Gauler R. Entomopathogenic nematodes. Annual Review of Entomology. 1993; 38: 181- 206.

Mwaitulo S, Haukeland S, Sæthre M-G, Laudisoit A, Maerere AP. First report of entomopathogenic nematodes from Tanzania and their virulence against larvae and adults of the banana weevil Cosmopolites sordidus (Coleoptera: Curculionidae). International Jour. Tropical Insect Science. 2011; 31 (3): 154-161 doi:10.1017/S1742758411000294

Amador M, Diego Molina CG, Parajeles E, Jiménez K, Uribe L. Utilización del nematodo entomopatógeno Heterorhabditis atacamensis CIA-NE07 en el control del picudo del banano Cosmopolites sordidus en condiciones in vitro. Agronomía Costarricense. 2015; 39 (3): 47-60

San-Blas E, Campos-Herrera R, Dolinski C, Monteiro C, Andaló V, Garrigós Leite L, et al. Entomopathogenic nematology in Latin America: A brief history, current research and future prospects. Journal of Invertebrate Pathology. 2019; 165: 22-45 doi.org/10.1016/j.jip.2019.03.010

Rodríguez MG. Entomopathogenic nematodes in Cuba: from laboratories to popular biological control agents for pest management in a developing country, in: Campos-Herrera, R. (Ed.), Nematode Pathogenesis of Insects and Other Pests - Ecology and Applied Technologies for Sustainable Plant and Crop Protection. Springer, Cham. 2015. pp. 343-364

Sánchez L, Rodríguez MG, Gómez L, Soler DM, Hernández MA, Castellanos L, et al. Desarrollo de una metodología para la reproducción artificial de nematodos entomopatógenos para el control de plagas en cafeto. PNCT, Desarrollo Sostenible de la Montaña. CODIGO, 1850 0703023. Informe final Proyecto - CENSA y Metodología Depositados en Centro de Derechos de Autor, Cuba, Numero de depósito: CENDA 09613/1852 2002. 2001. 60 pp.

Enrique R, Sánchez L, Rodríguez MG, Gómez L, del Valle Z. Dietas alternativas para la cría de G. mellonella. Influencia sobre el rendimiento - peso de larvas de Galleria mellonella y recobrado de juveniles infectivos. Centro Nacional de Derecho de Autor (CENDA). Numero de depósito: CENDA 2874-2006. Ciudad de la Habana, Cuba. 2006. 20 pp

Glazer I, Lewis EE. Bioassys for entomopathogenic nematodes. Pp. 229-247 En Bioassys of entomopathogenic Microbes and Nematodes. Navon A, Ascher KRS (Eds). CABI Publishing, UK. 2000.

Rosales LC, Suárez Z. Nematodos entomopatógenos como posibles agentes de control del gorgojo negro del plátano Cosmopolites sordidus (Germar 1824) (Coleoptera: Curculionidae). Bol Entomol Venez. 1998; 13(2):123-140.

Maniania NK, Ekesi S, Dolinski C. Entomopathogens routinely used in Pest Control Strategies: Orchards in Tropical Climate. En Microbial Control of Insect and Mite Pests. From theory to practice. Lawrence A. Lacey (Ed.). Pp 269-282. 2017. Academic Press. Elsevier Inc. http://dx.doi.org/10.1016/B978-0-12-803527-6.00018-4

Mwaniki Ndiritu Moses. Potential of entomopathogenic nematodes as a biological control and management tool for banana weevil (Cosmopolites sordidus) in banana orchards. [Thesis submitted in partial fulfillment of the requirements for the award of a degree of Master of Science in Crop Protection]. Department of Plant Science and Crop Protection, Faculty of Agriculture of the University of Nairobi. Kenya. 2016. 135 pp. Disponible en: https://pdfs.semanticschoola.org (acceso: 6 de julio, 2019)

Waturu CN, Wabule MN, Nguthi FN, Njinju SM. Field control of the banana weevil (Cosmopolites sordidus) using entomopathogenic nematodes. 2015. Disponible en https://www.researchgate.net/publication/267795896 (acceso: 6 de julio, 2019)

Schmitt AT, Gowen SR, Hague NGM. A baiting technique for the control of Cosmopolites sordidus Germar (Coleoptera: Curculionidae) by Steinernema carpocapsae (Nematoda: Steinernematidae). Nematropica. 1992; 22: 159-163

Smith D. Banana weevil borer control in south-eastern Queensland. Australian Journal of Experimental Agriculture. 1995; 35: 165-72

Most read articles by the same author(s)

> >>