Evaluation of bean genotype for resistance to Bean golden yellow mosaic virus (BGYMV)

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Lidia Chang-Sidorchuk
Alexis Lamz-Piedra
Heidy González-Alvarez
Arianna Morales-Soto
Alejandro Mederos Ramírez
Yamila Martínez-Zubiaur

Abstract

The common bean (Phaseolus vulgaris L.) is one of the most important grain legumes for human consumption. In Cuba, there are different factors that limit its production, among which the bean golden yellow mosaic disease, caused mainly by the begomovirus Bean golden yellow mosaic virus (BGYMV). Genetic improvement for resistance to this virus is one of the most effective strategies for disease control. The objective of this work is to evaluate the behavior of bean genotypes against the bean golden yellow mosaic disease and the identification of the resistance gene bgm-1 using the codominant SCAR marker S2 in genotypes from the INCA working collection. The severity of the viral symptoms was evaluated in each genotype in flowering stage according to the scale proposed by CIAT (1991). DNA extraction was performed on 61 genotypes. From the PCR analysis with the S2 primers it was possible to identify 19 genotypes with presence of the bgm-1 gene in recessive state, which confers the resistance to the BGYMV. 32 genotypes with 1 or 2 symptoms degree caused by begomovirus were detected, without the presence of the bgm-1 recessive gene. The results of this work constitute a great contribution to the common bean improvement program in Cuba, due to the fact that analyzed genotypes are materials from the genetic improvement programs of the region and from cultivars that belong to national production and constitute new resistance sources.

Article Details

How to Cite
Chang-Sidorchuk, L., Lamz-Piedra, A., González-Alvarez, H., Morales-Soto, A., Mederos Ramírez, A., & Martínez-Zubiaur, Y. (2021). Evaluation of bean genotype for resistance to Bean golden yellow mosaic virus (BGYMV). Revista De Protección Vegetal, 36(2). Retrieved from https://revistas.censa.edu.cu/index.php/RPV/article/view/1146
Section
SHORT COMMUNICATIONS

References

Calero-Hurtado A, Díaz YP, Rodriguez E, Viciedo D, Calzada K. Efecto de la aplicación asociada entre Rhizobium leguminosarum y microorganismos eficientes sobre la produccióndel frijol común. Ciencia y tecnología agropecuearia. 2019; 20(2).

Echemendía A, Ramos González P, Peral Pérez R, Porras González Á, González Arias GA. Selección de genotipos de frijol común (Phaseolus vulgaris L.) resistentes al virus del mosaico dorado amarillo del frijol (BGYMV) por hibridación de ácidos nucleicos. Fitosanidad. 2007;11(4):3-11.

Chang-Sidorchuk L, Alvarez HG, Martínez Zubiaur Y. Begomoviruses infecting common beans (Phaseolus vulgaris L.) in production areas in Cuba. Span. J. Agric. Res. 2018;16(2):23.

Galvez GE, Morales FJ. Whitefly-transmitted viruses. En: Bean Production Problems in the Tropics. 2nd ed. H.F. Schwartz and M. A. Pastor-Corrales, eds. Centro Internacional de Agricultura Tropical, Cali, Colombia. 1989. p. 379-406

Blanco N, Lastres N, Bencomo I. Incidencia de las enfermedades virosas del frijol en Cuba. Ciencias de la Agricultura [Cuba]. 1984;19:21-22.

de la Fé Montenegro CF, Lamz Piedra A, Cárdenas Travieso RM, Hernández Pérez J. Respuesta agronómica de cultivares de frijol común (Phaseolus vulgaris L.) de reciente introducción en Cuba. Cultivos Tropicales. 2016;37 (2): 102-107

Urrea CA, Miklas PN, Beaver JS, Riley RH. A codominant RAPD marker useful for indirect selection of BGMV resistance in common bean. J. Amer. Soc. Hort. Sci. 1996;121(6):1035-1039

Blair MW, Lina MR, Pedraza F, Morales F, Beeb S. Genetic mapping of the bean golden yellow mosaic geminivirus resistance gene bgm-1 and linkage with potyvirus resistance in common bean (Phaseolus vulgaris L.). Theor Appl Genet. 2007;114:261-271.

Hernández A, Pérez J, Bosh D, Castro N. Clasificación de los suelos de Cuba. 2015. Ediciones INCA, Mayabeque, Cuba. 1025, 93p. ISBN 978-959-7023-77-7

Faure B, Benítez R, García A, Ortega L. Manual para la producción sostenible del frijol común. Artemisa, Cuba. Instituto de Investigaciones de Granos. 2017; 114 p.

Permingeat HR, Romagnoli MV, Vallejos RH. A simple method for isolating high yield and quality DNA from cotton (Gossypium hirsutum L.) leaves. Plant Mol. Biol. Rep. 1998; 16: 1-6

CIAT: Sistema estándar para la evaluación de germoplasma de frijol, Centro Internacional de Agricultura Tropical, Cali, Colombia, 1991.

Beebe SE, Ochoa I, Skroch P, Nienhuis J, Tivang J. Genetic diversity among common bean breeding lines developed for Central America. Crop Sci. 1995; 35(4):1178–1183.

Singh SP, Morales FJ, Miklas PN, Terán H. Selection for bean golden mosaic resistance in intra- and interracial vean populations. Crop. Sci. 2000; 40:1565–1572.

Acevedo M, Molina A, Angel JC, Muñoz C, Beaver J. Inheritance of normal pod development in bean golden yellow mosaic resistant common bean. J Am Soc Hort Sci. 2004;129(4):549–552.

Miklas PN, Delorme R, Stone V, Daly MJ, Stavely JR, Basset MJ, et al. Bacterial, fungal and viral disease resistance loci mapped in a recombinant inbred common bean population (“Dorado”/XAN 176). J Am Soc Hort Sci. 2000; 125:476–481

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