In vitro compatibility between Rhizobium sp. (CIAT 899) and strains of Trichoderma asperellum Samuels, Lieckfeldt & Nirenberg and Pochonia chlamydosporia (Kamyschko ex Barron and Onions) Zare Gams

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Danay Ynfante Martinez
Ivonne González Marquetti
Belkis Peteira Delgado-Oramas
Jersys Arévalo Ortega
Rolisbel Alfonso de la Cruz
Benedicto Martínez Coca
Belkis Morales Mena
Susana Gorrita Ramirez
María Caridad Nápoles García

Abstract

The present work was developed with the objective of evaluating the in vitro compatibility of Trichoderma asperellum Samuels, Lieckfeldt & Nirenberg, and Pochonia chlamydosporia (Kamyschko ex Barron and Onions) Zare and Gams with Rhizobium biofertilizer (CIAT 899). The dual culture technique was used to determine compatibility by evaluating antibiosis (prior to their contact with the Rhizobium colony; at 48 hours and ten days) and competition for space (according to the grade scale referred to by Bell and the calculation of the percentage of the radial growth inhibition). Prior to the contact, the colonies of T. asperellum and P. chlamydosporia showed an inhibitory effect with respect to the control; with no significant differences in the case of P. chlamydosporia. At 96 hours, the Trichoderma strains were in grade 1 on the scale referred to by Bell, while Pochonia was in grade 2 at 18 days. Compared with the control, the evaluated strains of Trichoderma did not show inhibition to Rhizobium at 96 hours; Pochonia showed a decreasing effect with 2.5 % inhibition at 18 days.

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Ynfante Martinez, D. ., González Marquetti, I. ., Delgado-Oramas, B. P. ., Arévalo Ortega, J. ., Alfonso de la Cruz, R. ., Martínez Coca, B. ., Morales Mena, B. ., Gorrita Ramirez, S. ., & Nápoles García, M. C. . (2022). In vitro compatibility between Rhizobium sp. (CIAT 899) and strains of Trichoderma asperellum Samuels, Lieckfeldt & Nirenberg and Pochonia chlamydosporia (Kamyschko ex Barron and Onions) Zare Gams. Revista De Protección Vegetal, 37(3), https://cu-id.com/2247/v37n3e06. Retrieved from https://revistas.censa.edu.cu/index.php/RPV/article/view/1274
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