Capsicum chinense Jacq, new host of tomato chlorotic spot virus (TCSV) in Cuba

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Yarai Acosta Romay
Daimi Doval Delsa
Ana Ibis Elizondo Silva
Julia Elena Almándoz Parrado
Elisa Javer Higginson

Abstract

The present study aimed at identifying the viral agent associated with orthotospovirus-like symptoms on hot chili habanero bell pepper (Capsicum chinense Jacq) plants growing in a protected cultivation system area in Caimito municipality, Artemisa province. For this purpose, a group of symptomatic samples were collected at this location in May 2022, and they were tested by Double Antibody Sandwich- Enzyme-Linked ImmunoSorbent Assay (DAS-ELISA) with specific antibodies for tomato chlorotic spot virus (TCSV). Seven samples with necrosis and necrotic ringspots were positive for the virus. Mechanical inoculation on Nicotiana glutinosa L. and tomato (Solanum lycopersicum L.) produced local necrotic lesions and systemic necrosis, respectively. Reverse transcription-PCR with TCSV species-specific primers produced a fragment of approximately 948 bp size that confirmed TCSV infection. This is the first evidence of the presence of orthotospovirus in C. chinense in Cuba. Several thrips specimens were collected from the foliage of symptomatic plants. The insects were morphologically identified as Thrips palmi Karni and Frankliniella cephalica (Crawford), two recognized vectors of orthotospoviruses, but not of TCSV. In Cuba, this viral species and its insect vectors are widely distributed in tomato and pepper production areas. Its presence in the hot chili habanero bell pepper crop is a high risk for its production and export and accentuates the need for continuous surveillance of the TCSV-vector-host pathosystem to minimize potential damages to economically important crops.

Article Details

How to Cite
1.
Acosta Romay Y, Doval Delsa D, Elizondo Silva AI, Almándoz Parrado JE, Higginson EJ. Capsicum chinense Jacq, new host of tomato chlorotic spot virus (TCSV) in Cuba. Rev. Protección Veg. [Internet]. 2024 Dec. 29 [cited 2025 Jun. 4];39:https://cu-id.com/2247/v39e12. Available from: https://revistas.censa.edu.cu/index.php/RPV/article/view/1349
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SHORT COMMUNICATION

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