The most feasible diagnostic technique for the detection of Leifsonia xyli subsp. xyli in Cuba

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Javier Delgado Padrón
Iliana Miranda Cabrera
Yaquelin Puchades Izaguirre

Abstract

The objective of this work was to identify the most feasible diagnostic technique to detect Leifsonia xyli subsp. xyli (Davis) Evtushenko in sugarcane in Cuba. A systematic review of papers published online included in peer-reviewed indexed journals was carried out from 2000 to 2023. The keywords used were “diagnosis of diseases in sugarcane”, “Leifsonia xyli subsp. xyli”, “ratoon stunting disease” and their Spanish translation. The inclusion criterion was to take those papers that at least used one diagnostic technique for the bacterium detection that showed data on its sensitivity and/or specificity, with which a meta- analysis was carried out. A forest plot was created according to odds ratios and 95 % confidence intervals. Twelve techniques were identified for the diagnosis of ratoon stunting disease. The greatest accuracy is obtained with serological (ELISA) and molecular (HANnr and LAMP) techniques; TBIA and DOT BLOT are very similar to them, while IFA and IFM had a wider confidence interval. TBIA was identified as the most feasible diagnostic technique to detectf L. xyli subsp. xyli in sugarcane in Cuba.

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1.
Delgado Padrón J, Miranda Cabrera I, Puchades Izaguirre Y. The most feasible diagnostic technique for the detection of Leifsonia xyli subsp. xyli in Cuba. Rev. Protección Veg. [Internet]. 2025 Nov. 17 [cited 2026 Jun. 4];40:https://cu-id.com/2247/v40e24. Available from: https://revistas.censa.edu.cu/index.php/RPV/article/view/1459
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