Bordetella avium and Escherichia coli in broilers in Manabí province, Ecuador

Article Sidebar

PDF (Español (España)) EPUB (Español (España)) XML-JATS (Español (España)) HTML (Español (España))
Published: Aug 1, 2018
Keywords:
Avian Respiratory Complex, Bordetella avium, Escherichia coli, broilers

Main Article Content

Laura Monserrate De la Cruz-Veliz
Universidad Técnica de Manabí
Ivette Espinosa-Castaño
Centro Nacional de Sanidad Agropecuaria
Michel Báez-Arias
Centro Nacional de Sanidad Agropecuaria
Evelyn Lobo-Rivero
Centro Nacional de Sanidad Agropecuaria

Abstract

The Avian Respiratory Complex (ARC), is one of the most frequent problems in poultry. Its etiology is multifactorial and includes combinations of microorganisms, nutritional and environmental factors. Among the microbial agents, viruses, mycoplasmas and bacteria of the following genera and species: Ornithobacterium rhinotracheale, Bordetella avium, Pasteurella multocida and Escherichia coli, are reported. The objective of this study was to identify the presence of these bacteria in samples from broilers with symptoms compatible with the disease. To this end, 60 tracheal exudates of chickens belonging to four farms in Manabí province, Ecuador, were analyzed. The samples were identified by phenotypic (cultural, dye and biochemical) and genotypic tests by polymerase chain reaction (PCR). Five samples (8 %) tested positive for these bacteria, of which four corresponded to B. avium and one isolate to E. coli. The susceptibility profile revealed that both species were sensitive to ciprofloxacin and kanamycin, while they were resistant to amoxicillin and chloramphenicol. This study is the first evidence confirming the presence of these bacteria in broilers in Manabí province, Ecuador.

Article Details

How to Cite
1.
De la Cruz-Veliz LM, Espinosa-Castaño I, Báez-Arias M, Lobo-Rivero E. Bordetella avium and Escherichia coli in broilers in Manabí province, Ecuador. Rev. Salud Anim. [Internet]. 2018 Aug. 1 [cited 2024 Nov. 14];40(2). Available from: https://revistas.censa.edu.cu/index.php/RSA/article/view/971
Issue
Vol. 40 No. 2 (2018): mayo-agosto
Section
ARTÍCULOS ORIGINALES
National Center for Animal and Plant Health (CENSA)

References

Mosqueda M. El denominado Síndrome respiratorio del pollo de engorde. Sitio Argentino de Producción Animal. 2011. http://www.produccionanimal.com.ar

Kleven SH. Mycoplasmas; diagnóstico y monitoreo. XII Seminario Internacional de Patología y producción aviar en el Poultry Diagnostic and Research Center, College of Veterinary Medicine, University of Georgia, Athens, Georgia. 2010.

Koga Y. Enfermedades respiratorias inducidas por bacterias emergentes (Síndrome Respiratorio Bacteriano Aviar): diagnóstico y control. 2010. http://www.reinmark.com.

Barrientos R. Bacteriología en el complejo respiratorio de las aves. Servicio Nacional de Sanidad, Inocuidad y Calidad Agroalimentaria - Secretaría de Agricultura, Ganadería, Desarrollo Rural, Pesca y Alimentación (SENASICA-SAGARPA), México. 2011.

OIE. Código Sanitario para los animales terrestres. Capítulo 10.9. 2012

Poveda JB. Biochemical Characteristic in Mycoplasma identification. En: Methods in Molecular Biology. Vol. 104. Mycoplasma Protocols. Miles RJ, Nicholas RA. (eds). Humana Press Inc, Totowa, New Jersey. 1998. pp: 69-79.

Frey J. Mycoplasmas of animals. En: Molecular Biology and Pathogenecity of Mycoplasmas. Razin, S. y Herrmann, K. (eds.). Kluwer Academic/Plenum Publishers, New York. 2002. pp:73-90.

Zavala G. Practical use of molecular tools for epidemiological studies of current Mycoplasma respiratory problems. Memorias 57th WPDC/ANECA. 2008.

Beach M, Thompson S, Mutnick R, Brown L, Kettig G, Puffenbarger R. Bordetellaavium antibiotic resistance, novel enrichment culture, and antigenic characterization. Vet Microbiol 2012;160:189-96. doi:10.1016/j.vetmic.

Szabo R, Wehmann E, Magyar T. Antimicrobial susceptibility of Bordetella avium and Ornithobacterium rhinotracheale strains from wildland domesticated birds in Hungary. Acta Vet Hung. 2015; 63:413-24. doi:10.1556/004.2015.039

La Agencia Ecuatoriana de Aseguramiento de Calidad del Agro (AGROCALIDAD) http://simce.ambiente.gob.ec/2014.

De la Cruz L, Lobo E. Anticuerpos a Mycoplasmas ynoviae en pollos de engorde en granjas de la provincia de Manabí, Ecuador. Rev Salud Anim. 2013;35(3):206-209

Register K,Yersin AG. Analytical Verification of a PCR Assay for Identification of Bordetella avium. J Clin Microbiol. 2005;43(11):5567-5573.doi: 10.1128/JCM.43.11.5567-5573.

Chotar M, Vidova B, Godany A. Development of specific and rapid detection of bacterial pathogens in dairy products by PCR. Folia Microbiol. 2006;51:639-646.

Manual of Systematic Bacteriology Bergey. The Bacteroidetes,Spirochaetes, Tenericutes (Mollicutes), Acidobacteria, Fibrobacteres, Fusobacteria, Dictyoglomi, Gemmatimonadetes, Lentisphaerae, Verrucomicrobia, Chlamydiae, and Planctomycetes. Brown, D.R.; May, M.; Bradbury, J.M.; Johansson, K.E. and Neimark, H. 2nd edn, vol. 4, (edited by Krieg, Staley, Brown, Hedlund, Paster, Ward, Ludwig and Whitman). Springer, New York. 2010. pp. 574-644.

Blackall P, Doheny M. Isolation and characterisation of Bordetella avium and related species and an evaluation of their role in respiratory disease in poultry. Aust Vet J. 1987;64:235-239.

Hayashimoto N, Morita H, Yasuda M, Ishida T, Kameda S, Takakura A, et al. Prevalence of Bordetella hinzii in mice in experimental facilities in Japan. Res Vet Sci. 2012;93:624-626.

Noguera C, Infante D, León A, Fernández J, Rolo M, Avila I, Herrera A. Primer aislamiento e identificación del agente causal de Bordetelosis aviar y reproducción de la enfermedad en Venezuela. VetTrop. 2001;26(1):35-46.

Register B. Development of a PCR for Identification of Bordetella hinzii Avian Diseases. 2013;57(2):307-310. doi: 10.1637/10433-02212-ResNote.1

Harrington A, Castellanos J, Ziedalsky M, Clarridge JE, Cookson BT. Isolation of Bordetella avium and Novel Bordetella Strain from Patients with Respiratory Disease. Emerg Infect Dis. 2009;15(1):72-74.

Jackwood M, Saif Y. Bordetellosis (turkey coryza), 705-718. In Y.M. Saif (ed.), Diseases of poultry, 11th ed. Iowa StatePress, Ames, Iowa. 2003.

López Q. Manejo en el campo del complejo respiratorio. Soluciones. Informe técnico. Dpto. de Medicina y Zootecnia Avícola. Fac. de Medicina y Zootecnia. Universidad Nacional Autónoma de Mexico. 2012.

Cigoy L, Malena C, Terzolo R, Chacana A. Aislamiento de Bordetella avium en pollos de engorde en Argentina. Avian Dis. 2011;37:310-314.

Karen B, Randy E, Gwen E. Nordholm Comparison of Ribotyping and Restriction Enzyme Analysis for Interand Intraspecies Discrimination of Bordetella avium and Bordetella hinzii J Clin Microbiol. 2003;41(4):1512-1519.

Gi YL, Hye I, Gyun H, Min S. Prevalence and classification of pathogenic Escherichia coli isolated from fresh beef, poultry, and pork in Korea. 2009.

Piatti G, Mannini A, Balistreri M, Schito A. Virulence factors in urinary Escherichia coli strains: phylogenetic background and quinolone and fluoroquinolone resistance. J Clin Microbiol. 2008;6:480-487.

Rodríguez-Ángeles G. Principales características y diagnóstico de los grupos patógenos de Escherichia coli. 2002. pp. 464-475.

Martínez-Alesón S. Procesos respiratorios en aves jóvenes. Sitio Argentino de Producción Animal. 2010. http://www.produccion-animal.com.ar.

Abu-Basha EA, Gharaibeh SM, Thabet AM. In vitro susceptibility of resistant Escherichia coli field isolates to antimicrobial combinations. J Appl Poult Res. 2012;1:595-602. doi:10.3382/japr.2011-00500

Veterinary Investigation Diagnosis Analysis (VIDA). Annual Report on Data Recorded by APHA and Scotland’s Rural College on Animal and Bird Diagnostic Submissions in Great Britain. Animal and Plant Health Agency (APH). 2016. Available from: http://ahvla.defra.gov.uk/vet-gateway/surveillance/reports.htm

Most read articles by the same author(s)

> >> 

Similar Articles

You may also start an advanced similarity search for this article.