Physiological and cultural characterization and mycelial compatibility of Sclerotium sp. isolates from seven plant hosts

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Yanisia Duarte Leal
Benedicto Martínez Coca
Adalberto C. Café Filho
Luiz Eduardo Bassay Blum

Abstract

The objectives of the work were to culturally and physiologically characterize 15 isolates of Sclerotium sp. from beans (Phaseolus vulgaris L.), chickpea (Cicer arietinum L.), tomato (Solanum lycopersicum L.), taro [Colocasia esculenta (L.) Schott], garlic (Allium sativum L.), and ornamental plants [Neomarica caerulea (Ker Gawl.) Sprague and Hydrocotyle sp.], from Cuba and Brazil, under different lighting conditions and determine the presence of their Mycelial Compatibility Groups (GCM). For this, the inoculated Petri dishes containing Papa Dextrose Agar (PDA) culture medium were placed under three lighting regimes [constant darkness (OC), alternating light (LA), and constant light (LC)] at 28 ± 2°C. The mycelial growth diameter and the surface texture and color of the colonies were evaluated in each isolate, as well as the number, weight and diameter of sclerotia under each condition. Confrontation tests were used to determine the existence of GCM. The highest production of sclerotia was under LC, and the lowest under OC. Mycelial compatibility was observed between the colonies of isolates from the same host (No. 1 and 9 of C. arietinum and No. 5 and 6 of P. vulgaris) and between isolates from different hosts (P. vulgaris - S. lycopersicum and A. sativum - N. caerulea). Five GCM were obtained, GCM- I (No. 1 and 9), GCM-II (No. 5 and 6), GCM-III (No. 10 and 11), GCM-IV (No. 10 and 12), and GCM-V (No. 13 and 14).

Article Details

How to Cite
Duarte Leal, Y., Martínez Coca, B., Café Filho, A. C., & Bassay Blum, L. E. (2020). Physiological and cultural characterization and mycelial compatibility of Sclerotium sp. isolates from seven plant hosts. Revista De Protección Vegetal, 35(1). Retrieved from https://revistas.censa.edu.cu/index.php/RPV/article/view/1086
Section
ORIGINAL ARTICLES

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