In vitro biofumigant potential of three species of Brassicaceae for soil phytopathogen management

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Verónica Toledo Sampedro
Javier Martínez Pacheco
Angélica de la C. González Toledo

Abstract

The objective of this research was to evaluate the in vitro biofumigant effect of species of the family Brassicaceae on mycelial growth of phytopathogens in tobacco (Nicotiana tabacum L.). Three species were evaluated: Brassica juncea L. (Indian mustard), B. oleracea var. accephala L. (cabbage) and Sinapis alba L. (white mustard), which were tested against seven isolates (in parenthesis): Phytophthora nicotianae (2), Fusarium oxysporum (2), Fusarium solani (2), and Rhizoctonia solani (1). The Brassicas species, at a concentration of 2.5 g/L and 5 g/L container capacity (20 L), were crushed and placed in beakers with 600 mL of distilled water. Containers of 20 L were used as incubation chamber, in which the Petri dishes, without the lids, of all the isolates were placed in triplicate, and in the center, the beaker with distilled water and the plant material. The percentage of mycelial growth inhibition of each isolate was determined 7 days after incubating the containers at 27ºC in the dark. To determine viability of the isolates after the treatments with each of the biofumigants, the mycelium discs were transferred to fresh potato dextrose agar culture medium. Concentration increase of the species B. juncea, B. oleracea, and S. alba to 5 g/L, increased the percentage of the in vitro growth inhibition of P. nicotianae, F. oxysporum, F. solani and R. solani isolates. Except for the isolate of R. solani, the results obtained showed that B. juncea, B. oleraea and S. alba had fungistatic potentialities at concentrations of 5 g/L.

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How to Cite
Toledo Sampedro, V., Martínez Pacheco, J., & González Toledo, A. de la C. (2021). In vitro biofumigant potential of three species of Brassicaceae for soil phytopathogen management. Revista De Protección Vegetal, 35(3). Retrieved from https://revistas.censa.edu.cu/index.php/RPV/article/view/1099
Section
ORIGINAL ARTICLES

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