Molecular characterisation of ‘Candidatus Phytoplasma asteris’ and a Bean golden yellow mosaic virus isolate present in mixed infection in common bean in Cuba

Main Article Content

Loidy Zamora
Madelaine Quiñones
Karel Acosta
Bertha Piño
María Elena Santos
Jesús Méndez
Alicia Chávez
Norma Elena Leyva
Yaima Arocha

Abstract

Common bean fields of Havana and Mayabeque provinces, Cuba, were surveyed from October to March in 2011 to 2013 to examine symptoms associated with the mixed infections by phytoplasmas and begomoviruses. Total DNA was extracted from ninety-nine symptomatic and ten symptomless plants and used as a template in phytoplasma detection by nested PCR using universal primers that target the 16S rRNA gene, and in the PCR for amplification of the begomovirus N-terminal region of the Rep protein, the intergenic region, and the N-terminal region of the coat protein. The sequences of phytoplasmas and begomoviruses obtained were compared with those of reference at the GenBank by BLASTn. Of the total samples collected, 72.72 % were positive to these infections. Of these, phytoplasmas were detected in 11.11 %, begomoviruses in 17.17 %, and 44 % showed a mixed infection by both pathogens. RFLP and phylogeny analyses confirmed the identification of the phytoplasma as a member of the group 16SrI ‘Candidatus Phytoplasma asteris’, subgroup 16SrI-B. Sequence analysis revealed that the begomovirus coexisting with the 16SrI phytoplasma in common bean plants was the Bean Golden Yellow Mosaic Virus (BGYMV). The sequence of the A component of the present BGYMV showed 98 % similarity with that of a previously BGYMV isolated in Cuba (AJ544531), and its B component was closely related to that of the clade of the American isolate BGYMV-US (DQ119825).

Article Details

How to Cite
Zamora, L., Quiñones, M., Acosta, K., Piño, B., Santos, M. E., Méndez, J., Chávez, A., Leyva, N. E., & Arocha, Y. (2021). Molecular characterisation of ‘Candidatus Phytoplasma asteris’ and a Bean golden yellow mosaic virus isolate present in mixed infection in common bean in Cuba. Revista De Protección Vegetal, 36(3). Retrieved from https://revistas.censa.edu.cu/index.php/RPV/article/view/1169
Section
ORIGINAL ARTICLES

References

Bellucci E, Bitocchi E, Rau D, Rodriguez M, Biagetti E, Giardini A, et al. Genomics of origin, domestication and evolution of Phaseolus vulgaris In: Tuberosa R, Graner A, Frison E., Eds. Genomics of Plant Genetic Resources. Dordrecht: Springer. 2014:483-507.

Hernández Morales A (Coordinación y Revisión General). La cadena de valor del frijol común en Cuba. Estudio de su situación en siete municipios de las provincias de Sancti Spíritus y Villa Clara. Agosto, 2016. Editado por: Agrocadenas. Programa de apoyo al fortalecimiento de cadenas agroalimentarias a nivel local. 175 págs. ISBN: 978-959-296-045-9

Hernández-Ochandía D, Rodríguez MG, Holgado R. Nematodos parásitos que afectan Phaseolus vulgaris L. en Latinoamérica y Cuba: especies, daños y tácticas evaluadas para su manejo. Rev. Protección Veg. 2018; 33(3):1-17.

Llanes V, González E, Mederos D, Rodríguez H. Percepción de los productores de Phaseolus vulgaris L. acerca de las necesidades de capacitación sobre las plagas. Rev. Protección Veg. 2019; 34(1):113-118.

Chang-Sidorchuk L, González-Álvarez H, Martínez-Zubiaur Y. Begomoviruses infecting common beans (Phaseolus vulgaris L.) in production areas in Cuba. Spanish Journal of Agricultural Research. 2018; 16(2):1006.

Zamora L, Acosta K, Martínez Y. First report of 'Candidatus Phytoplasma asteris' (16SrI group) affecting common bean in Cuba. New Disease Reports. 2012; 25:4. [http://dx.doi.org/10.5197/j.2044-0588.2012.025.004]

Stanley J, Bisaro DM, Briddon RW, Brown J, Fauquet CM, Harrison BD, et al. Family Geminiviridae. En: C. M. Fauquet, M. A. Mayo, J. Maniloff, U. Desselberger y L. A. Ball (eds). Virus Taxonomy: Eighth Report of the International Committee on Taxonomy of Viruses. 2005.. 301-326 págs. San Diego: Elsevier Academic Press.

Leyva R, Quiñones M, Piñol B, Piloto E, Acosta K. Detection of mixed infection of ‘Candidatus phytoplasma sp.’ and begomoviruses sp affecting soybean crop in the eastern region of Cuba. Rev. Protección Veg. 2019; 34(2):10. E-ISSN: 2224-4697

Morales FJ, Castaño M. Enfermedades virales del Frijol Común en América Latina. Centro Internacional de Agricultura Tropical. Unidad de Virología (CIAT); Palmira, Colombia. 2008. 86 págs.

Martínez-Zubiaur Y, Chang-Sidorchuk L, González-Álvarez H. Begomoviruses in Cuba: Brief History and Current Status. In S. Saxena, A. K. Tiwari (eds.), Begomoviruses: Occurrence and Management in Asia and Africa. Springer Nature Singapore. 2017:315-329.

Blanco N, Faure B. Situación actual del mosaico dorado del frijol en el Caribe, Cuba. El mosaico dorado del frijol, avances de investigación. Centro Internacional de Agricultura Tropical (CIAT), Cali, Colombia. 1994:82-89.

Echemendía AL, Ramos PL, Villarreal N, Martínez AK, González G, Morales FJ. Caracterización del virus del mosaico amarillo dorado del frijol en Cuba. Fitosanidad. 2010; 14:11-17.

Leyva RM, Quiñones ML, Acosta KI, Piñol B, Xavier CD, Zerbini FM. First report of Tobacco leaf curl Cuba virus infecting common bean in Cuba. New Disease Reports. 2016; 33:17. http://dx.doi.org/10.5197/j.2044-0588.2016.033.017

Bertaccini A. Phytoplasma research between past and future: what directions? Phytopathogenic Mollicutes. 2015; 5 (1): S1-S4.

Mou HQ, Zhu SF, Xu X, Zhao WJ. An overview of research on phytoplasma-induced diseases. Plant Protect. 2011; 37:17-22.

Acosta K, Quiñones M, Pérez-López E. Diversity of Phytoplasmas in Cuba, Their Geographic Isolation and Potential Development of Management Strategies. CY. Olivier, et al. (eds.), Sustainable Management of Phytoplasma Diseases in Crops Grown in the Tropical Belt, Sustainability in Plant and Crop Protection 12. 2019:87-103. https://doi.org/10.1007/978-3-030-29650-6_4

Arocha Y, Piñol B, Picornell B, Almeida R, Jones P. Broad bean and sweet pepper: two new hosts associated with Candidatus Phytoplasma asteris (16SrI phytoplasma group) in Cuba. Plant Pathology. 2007; 56:345.

Singh J, Singh A, Kumar P, Rani A, Baranwal VK, Sirohi A. First report of mixed infection of phytoplasmas and begomoviruses in eggplant in India. Phytopathogenic Mollicutes. 2015; 5(1s):S97-S98.

Venkataravanappa V, Prasanna HC, Lakshminarayana CN, Reddy MK. Molecular detection and characterization of phytoplasma in association with begomovirus in eggplant. Acta virológica. 2018; 2(3):246-258.

Lebsky V, Hernández-González J, Arguello-Astorga G, Cardenas-Conejo Y, Poghosyan A. Detection of phytoplasmas in mixed infection with begomoviruses: a case study of tomato and pepper in Mexico. Bulletin of Insectology.2011; 64:55-56.

Gungoosingh-Bunwaree A, Contaldo N, Mejia JF, Paltrinieri S, Beni Madhu SP, Bertaccini A. Phytoplasma and virus diseases on tomato in Mauritius. Australasian Plant Pathology. 2012; 42:659-665.

Swarnalatha P, Krishna M. Duplex PCR for simultaneous detection of Begomovirus and Phytoplasma from naturally infected tomato. Pest Management in Horticultural Ecosystems. 2014; 20:59-68.

Zamora L. Detection and identification of Ca. Phytoplasmas sp. and begomovirus on Phaseolus vulgaris L. in Cuba. Rev. Protección Veg. 2014; 29(3):236.

Doyle JJ, Doyle JL. Isolation of plant DNA from fresh tissue. Focus. 1990; 12:13-15.

Gundersen DE, Lee IM. Ultrasensitive detection of phytoplasmas by nested-PCR assays using two universal primer pairs. Phytopathologia Mediterranea. 1996; 35:144-151.

Argüello-Astorga G, Ruiz-Medrano R. An iteron-related domain is associated to Motif 1 in the replication proteins of geminiviruses: identification of potential interacting amino acid-base pairs by a comparative approach. Archives of Virology. 2001; 146:1465-1485.

Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. Journal of Molecular Biology. 1990; 215:403-410.

Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony. Methods. Molecular Biology and Evolution. 2011; 28:2731-2739.

Moreira L, Villalobos W, Saborío RG, Garita L, Castro-Robleda S, Romero-Cano J, et al. A phytoplasma associated with ‘amachamiento’ disease of dry common bean in Costa Rica. Plant Pathol. New Disease Reports. 2009; 19:31.

Rodríguez-Pardina PE, Zerbini FM, Ducasse DA. Genetic Diversity of Begomoviruses infecting soybean, bean and associated weeds in Northwestern Argentina. Fitopatol. Bras. 2006; 31:342-348.

Fernandes FR, Cruz ARR, Faria JC, Zerbini FM, Aragao FJL. Three distinct begomoviruses associated with soybean in central Brazil. Arch. Virol. 2009; 154:1567-1570.

Mendez-Lozano J, Perea-Araujo LL, Ruelas-Ayala RD, Leyva-Lopez NE, Mauricio-Castillo JA, Arguello-Astorga GR. A begomovirus isolated from stunted and chlorotic soybean plants in Mexico is a new strain of Rhynchosia golden mosaic virus. Plant Dis. 2006; 90:972.

Martínez-Zubiaur Y, Quiñones M, Fonseca D, Potter J, Maxwell DP. First report of Tomato yellow leaf curl virus associated with beans, Phaseolus vulgaris, in Cuba. Plant Dis. 2002; 86;814.

Morales FL, Anderson PK. The emergence and dissemination of whitefly-transmitted geminivirus in Latin America. Arch Virol. 2001; 146:415-441.

Hanley-Bowdoin L, Settlage SB, Orozco BM, Nagar S, Robertson D. Geminiviruses: Models for plant DNA replication, transcription and cell cycle regulation. Critical Review in Biochemical and Molecular Biology. 2000; 35:105-140.

Firrao G, Garcia-Chapa M, Marzachì C. Phytoplasmas: genetics, diagnosis and relationships with the plant and insect host. Frontiers in Bioscience. 2007; 12:1353-1375.

Most read articles by the same author(s)

> >>