Effects of Trichoderma asperellum Samuels, Lieckfeldt & Nirenberg and Azofert ® on growth and development of Phaseolus vulgaris L.

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Ivonne González-Marquetti
Danay Ynfante-Martínez
Susana Gorrita
Belkis Morales-Mena
María Caridad Nápoles
Belkis Peteira Delgado-Oramas
Benedicto Martínez-Coca

Abstract

The aim of this work was to evaluate the effect of Trichoderma asperellum Samuels, Lieckfeldt & Nirenberg (Ta. 13, Ta. 78 and Ta. 90) combined with Azofert® on growth and development of Phaseolus vulgaris L. (cv. 'BAT-304') under semi-controlled conditions. Evaluations were carried out in 32-day-old plants sown at two times: 1) plant sowing and soil inoculation with Trichoderma at the same time, 2) plant sowing seven days after soil inoculation with the fungus. The plant height, root length, number of leaves, number of flowers, number of tendrils and pods, fresh and dry weight of plant aerial parts and roots, Trichoderma endophytic capacity in roots, nodulation effectiveness by Rhizobium and the induction of peroxidase enzymes (POD), polyphenoloxidases (PPO), and phenylalanine ammonium lyases (PAL) were determined. All results were contrasted according to simple analysis of variance followed by the least significant difference test. The co-inoculation of Ta. 13 and Ta. 78 with Azofert® had a synergist effect on growth and defense enzymes stimulation of P. vulgaris. The ability of the T. asperellum strains studied to endophytically colonize the roots of P. vulgaris (cv. 'BAT-304') and their compatibility with Azofert® were verified, under controlled conditions. In general, the treatments to which T. asperellum was applied from the moment of sowing presented the best values of the growth and development parameters evaluated. The co-inoculation of Ta. 13 and Ta. 78 with Azofert® had a synergistic effect on growth stimulation and expression of defense enzymes PAL, PPO and POD of P. vulgaris.

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How to Cite
González-Marquetti, I., Ynfante-Martínez, D., Gorrita, S., Morales-Mena, B., Nápoles, M. C., Delgado-Oramas, B. P., & Martínez-Coca, B. (2021). Effects of Trichoderma asperellum Samuels, Lieckfeldt & Nirenberg and Azofert ® on growth and development of Phaseolus vulgaris L. Revista De Protección Vegetal, 36(3). Retrieved from https://revistas.censa.edu.cu/index.php/RPV/article/view/1171
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ORIGINAL ARTICLES

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